ArticleInfection and immunity2014
Identification of cell surface-exposed proteins involved in the fimbria-mediated adherence of enteroaggregative Escherichia coli to intestinal cells.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- EPEC autotransporter adhesin (Eaa): a novel adhesin identified in atypical enteropathogenicFrontiers in cellular and infection microbiology · 2025Article
- Aggregative adherence fimbriae form compact structures as seen by SAXS.Scientific reports · 2023Article
- Probing gut-brain links in Alzheimer's disease with rifaximin.Alzheimer's & dementia (New York, N. Y.) · 2022Article
- Redefining enteroaggregative Escherichia coli (EAEC): Genomic characterization of epidemiological EAEC strains.PLoS neglected tropical diseases · 2020Article
- Aggregative Adherence Fimbriae II of Enteroaggregative Escherichia coli Are Required for Adherence and Barrier Disruption during Infection of Human Colonoids.Infection and immunity · 2020Article
- Identification and characterisation of enteroaggregative Escherichia coli subtypes associated with human disease.Scientific reports · 2020Article
- Shiga Toxin-Producing and Enteroaggregative Escherichia coli in Animal, Foods, and Humans: Pathogenicity Mechanisms, Detection Methods, and Epidemiology.Current microbiology · 2020Review
- The Not so Good, the Bad and the Ugly: Differential Bacterial Adhesion and Invasion Mediated byMicroorganisms · 2020Article
- Oxygen and contact with human intestinal epithelium independently stimulate virulence gene expression in enteroaggregative Escherichia coli.Cellular microbiology · 2019Article
- TLR4 Participates in the Inflammatory Response Induced by the AAF/II Fimbriae From EnteroaggregativeFrontiers in cellular and infection microbiology · 2019Article
- Adhesion of EnteroaggregativeBio-protocol · 2018Article
- Galactooligosaccharide supplementation provides protection against Citrobacter rodentium-induced colitis without limiting pathogen burden.Microbiology (Reading, England) · 2018Article
- EnteroaggregativemBio · 2017Article
- The Role of Fibronectin in the Adherence and Inflammatory Response Induced by EnteroaggregativeFrontiers in cellular and infection microbiology · 2016Article
- Structural insight into host recognition by aggregative adherence fimbriae of enteroaggregative Escherichia coli.PLoS pathogens · 2014Article
- Epidemiology and clinical manifestations of enteroaggregative Escherichia coli.Clinical microbiology reviews · 2014Review
- Long polar fimbriae participates in the induction of neutrophils transepithelial migration across intestinal cells infected with enterohemorrhagic E. coli O157:H7.Frontiers in cellular and infection microbiology · 2014Article
- Participation of integrin α5β1 in the fibronectin-mediated adherence of enteroaggregative Escherichia coli to intestinal cells.BioMed research international · 2014Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Fimbria-mediated adherence to the intestinal epithelia is a key step in enteroaggregative Escherichia coli (EAEC) pathogenesis. To date, four fimbriae have been described for EAEC; aggregative adherence fimbria II (AAF/II) is the most important adherence factor for EAEC prototype strain 042. Previously, we described results showing that extracellular matrix (ECM) components might be involved in the recognition of AAF/II fimbriae by intestinal cells. In this study, we sought to identify novel potential receptors on intestinal epithelial cells recognized by the AAF/II fimbriae. Purified AafA-dsc protein, the major subunit of AAF/II fimbriae, was incubated with a monolayer of T84 cells, cross-linked to the surface-exposed T84 cell proteins, and immunoprecipitated by using anti-AafA antibodies. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of cellular proteins bound to AafA-dsc protein identified laminin (previously recognized as a potential receptor for AAF/II) and cytokeratin 8 (CK8). Involvement of the major subunit of AAF/II fimbriae (AafA protein) in the binding to recombinant CK8 was confirmed by adherence assays with purified AAF/II fimbriae, AafA-dsc protein, and strain 042. Moreover, HEp-2 cells transfected with CK8 small interfering RNA (siRNA) showed reduced 042 adherence compared with cells transfected with scrambled siRNA as a control. Adherence of 042 to HEp-2 cells preincubated with antibodies against ECM proteins or CK8 was substantially reduced. Altogether, our results supported the idea of a role of CK8 as a potential receptor for EAEC.
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