ArticleJournal of endocrinological investigation2014
Orexin A-mediated stimulation of 3β-HSD expression and testosterone production through MAPK signaling pathways in primary rat Leydig cells.
Article in Journal of endocrinological investigation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 24 citations in OpenAlex.
- A Comparative Analysis of Orexins in the Physio-Pathological Processes of the Male Genital Tract: New Challenges? A Review.Veterinary sciences · 2024Review
- A Review on the Impact of Oxidative Stress and Medicinal Plants on Leydig Cells.Antioxidants (Basel, Switzerland) · 2023Review
- Orexin/Hypocretin System Dysfunction in ESSENCE (Early Symptomatic Syndromes Eliciting Neurodevelopmental Clinical Examinations).Neuropsychiatric disease and treatment · 2022Article
- Endocrinopathies and Male Infertility.Life (Basel, Switzerland) · 2021Review
- Myotubularin related protein 7 is essential for the spermatogonial stem cell homeostasis via PI3K/AKT signaling.Cell cycle (Georgetown, Tex.) · 2019Article
- Reactive oxygen species and male reproductive hormones.Reproductive biology and endocrinology : RB&E · 2018Review
- Potential role of orexin A binding the receptor 1 for orexins in normal and cryptorchid dogs.BMC veterinary research · 2018Article
- Biochemical and Clinical Impact of Organic Uremic Retention Solutes: A Comprehensive Update.Toxins · 2018Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOrexin A (ORA) regulates food intake, energy metabolism, gastrointestinal and reproductive functions.
aimThe purpose of this study was to demonstrate whether the expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) and testosterone was stimulated by ORA and mediated through mitogen-activated protein kinases (MAPK) in rat Leydig cells.
methodsPrimary Leydig cells were isolated from male rat testes, cultured, and treated with ORA under various conditions.
resultsOrexin receptor 1 (OX (1) R) mRNA, but not orexin receptor 2 mRNA, was detected in primary Leydig cells. ORA up-regulated the expression of OX( 1) R mRNA and protein in a dose-responsive manner and increased the phosphorylation of extracellular receptor kinase 1/2 (ERK1/2) and p38 MAPK levels, but did not affect the phosphorylation of the JNK MAPK. Phosphorylation of ERK1/2 and p38 MAPKs by ORA was blocked with U0126 and SB203580 inhibitors, respectively. An OX(1)R-specific inhibitor, SB334867, also blocked the phosphorylation of ERK1/2 and p38 by ORA. Inhibitor treatment also blocked 3β-HSD expression and testosterone production.
conclusionsThese results demonstrate that ORA activation of OX(1)R up-regulates 3β-HSD expression and testosterone production via the ERK1/2 and p38 MAPKs signaling pathways in primary rat Leydig cells.
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