ArticleThe Journal of biological chemistry2014
(-)-Epicatechin gallate (ECG) stimulates osteoblast differentiation via Runt-related transcription factor 2 (RUNX2) and transcriptional coactivator with PDZ-binding motif (TAZ)-mediated transcriptional activation.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Effects of Resveratrol, Curcumin and Quercetin Supplementation on Bone Metabolism-A Systematic Review.Nutrients · 2022Pooled it
- Effects of cocoa extract supplementation and multivitamin/multimineral supplements on self-reported fractures in the Cocoa Supplement and Multivitamins Outcomes Study randomized clinical trial.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2025Trial
- Flavonoids ofPreventive nutrition and food science · 2026Article
- Investigating the Osteoregenerative Properties ofJournal of functional biomaterials · 2025Article
- Role of FGF2 in promoting osteogenic differentiation for craniofacial bone regeneration.Regenerative engineering and translational medicine · 2025Article
- Mechanistic Exploration ofPharmaceuticals (Basel, Switzerland) · 2024Article
- Cell signaling and transcriptional regulation of osteoblast lineage commitment, differentiation, bone formation, and homeostasis.Cell discovery · 2024Review
- The potential application of fermented tea as a drink for regulating bone mass.Frontiers in pharmacology · 2024Review
- Potential mechanism of tea for treating osteoporosis, osteoarthritis, and rheumatoid arthritis.Frontiers in medicine · 2024Review
- Microenvironmental mechanoactivation through Yap/Taz suppresses chondrogenic gene expression.Molecular biology of the cell · 2023Article
- Epicatechin gallate promotes vascularization in co-culture of human osteoblasts and outgrowth endothelial cells.Experimental biology and medicine (Maywood, N.J.) · 2023Article
- Functionalization with Polyphenols of a Nano-Textured Ti Surface through a High-Amino Acid Medium: A Chemical-Physical and Biological Characterization.Nanomaterials (Basel, Switzerland) · 2022Article
- Polyphenols: Bioavailability, Microbiome Interactions and Cellular Effects on Health in Humans and Animals.Pathogens (Basel, Switzerland) · 2022Review
- Gone Caving: Roles of the Transcriptional Regulators YAP and TAZ in Skeletal Development.Current osteoporosis reports · 2020Review
- Compounds isolated fromSaudi journal of biological sciences · 2020Article
- Article
- Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells.International journal of molecular medicine · 2020Article
- Polydatin improves osteogenic differentiation of human bone mesenchymal stem cells by stimulating TAZ expression via BMP2-Wnt/β-catenin signaling pathway.Stem cell research & therapy · 2020Article
- Article
- Role of YAP/TAZ in Cell Lineage Fate Determination and Related Signaling Pathways.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Osteoporosis is a degenerative bone disease characterized by low bone mass and is caused by an imbalance between osteoblastic bone formation and osteoclastic bone resorption. It is known that the bioactive compounds present in green tea increase osteogenic activity and decrease the risk of fracture by improving bone mineral density. However, the detailed mechanism underlying these beneficial effects has yet to be elucidated. In this study, we investigated the osteogenic effect of (-)-epicatechin gallate (ECG), a major bioactive compound found in green tea. We found that ECG effectively stimulates osteoblast differentiation, indicated by the increased expression of osteoblastic marker genes. Up-regulation of osteoblast marker genes is mediated by increased expression and interaction of the transcriptional coactivator with PDZ-binding motif (TAZ) and Runt-related transcription factor 2 (RUNX2). ECG facilitates nuclear localization of TAZ through PP1A. PP1A is essential for osteoblast differentiation because inhibition of PP1A activity was shown to suppress ECG-mediated osteogenic differentiation. Taken together, the results showed that ECG stimulates osteoblast differentiation through the activation of TAZ and RUNX2, revealing a novel mechanism for green tea-stimulated osteoblast differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.