ArticleAntimicrobial agents and chemotherapy2014
Temporins A and B stimulate migration of HaCaT keratinocytes and kill intracellular Staphylococcus aureus.
Article in Antimicrobial agents and chemotherapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 72 citations in OpenAlex.
- Antimicrobial Peptides for Skin Wound Healing.Biomolecules · 2025Review
- Article
- Antimicrobial and antitumor properties of anuran peptide temporin-SHf induce apoptosis in A549 lung cancer cells.Amino acids · 2024Article
- Temporins: Multifunctional Peptides from Frog Skin.International journal of molecular sciences · 2023Review
- The Skin Microbiome: Current Landscape and Future Opportunities.International journal of molecular sciences · 2023Review
- Characterization and mechanism of action of amphibian-derived wound-healing-promoting peptides.Frontiers in cell and developmental biology · 2023Review
- Amphibian-derived wound healing peptides: chemical molecular treasure trove for skin wound treatment.Frontiers in pharmacology · 2023Review
- Wound healing mechanism of antimicrobial peptide cathelicidin-DM.Frontiers in bioengineering and biotechnology · 2022Article
- Marine Fungal Cerebroside Flavuside B Protects HaCaT Keratinocytes againstMarine drugs · 2021Article
- Temporin G, an amphibian antimicrobial peptide against influenza and parainfluenza respiratory viruses: Insights into biological activity and mechanism of action.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Cathelicidin ΔPb-CATH4 derived from Python bivittatus accelerates the healing of Staphylococcus aureus-infected wounds in mice.Amino acids · 2021Article
- The Antimicrobial Peptide Temporin G: Anti-Biofilm, Anti-Persister Activities, and Potentiator Effect of Tobramycin Efficacy AgainstInternational journal of molecular sciences · 2020Article
- Functional Characterization of Temporin-SHe, a New Broad-Spectrum Antibacterial and Leishmanicidal Temporin-SH Paralog from the Sahara Frog (International journal of molecular sciences · 2020Article
- Design, Engineering and Discovery of Novel α-Helical and β-Boomerang Antimicrobial Peptides against Drug Resistant Bacteria.International journal of molecular sciences · 2020Review
- Therapeutic potential of a designed CSαβ peptide ID13 in Staphylococcus aureus-induced endometritis of mice.Applied microbiology and biotechnology · 2020Article
- In vitro and intracellular activities of frog skin temporins against Legionella pneumophila and its eukaryotic hosts.Scientific reports · 2020Article
- The Bactericidal Activity of Temporin Analogues Against Methicillin ResistantInternational journal of molecular sciences · 2019Article
- Article
- Peptides for Skin Protection and Healing in Amphibians.Molecules (Basel, Switzerland) · 2019Review
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The growing number of microbial pathogens resistant to available antibiotics is a serious threat to human life. Among them is the bacterium Staphylococcus aureus, which colonizes keratinocytes, the most abundant cell type in the epidermis. Its intracellular accumulation complicates treatments against resulting infections, mainly due to the limited diffusion of conventional drugs into the cells. Temporins A (Ta) and B (Tb) are short frog skin antimicrobial peptides (AMPs). Despite extensive studies regarding their antimicrobial activity, very little is known about their activity on infected cells or involvement in various immunomodulatory functions. Here we show that Tb kills both ATCC-derived and multidrug-resistant clinical isolates of S. aureus within infected HaCaT keratinocytes (80% and 40% bacterial mortality, respectively) at a nontoxic concentration, i.e., 16 μM, whereas a weaker effect is displayed by Ta. Furthermore, the peptides prevent killing of keratinocytes by the invading bacteria. Further studies revealed that both temporins promote wound healing in a monolayer of HaCaT cells, with front speed migrations of 19 μm/h and 12 μm/h for Ta and Tb, respectively. Migration is inhibited by mitomycin C and involves the epidermal growth factor receptor (EGFR) signaling pathway. Finally, confocal fluorescence microscopy indicated that the peptides diffuse into the cells. By combining antibacterial and wound-healing activities, Ta and Tb may act as multifunctional mediators of innate immunity in humans. Particularly, their nonendogenous origin may reduce microbial resistance to them as well as the risk of autoimmune diseases in mammals.
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