Evidence map›Paper›PMID 24512720›Full record

ArticleEpidemiology and infection2014

Distribution of Escherichia coli strains harbouring Shiga toxin-producing E. coli (STEC)-associated virulence factors (stx1, stx2, eae, ehxA) from very young calves in the North Island of New Zealand.

H Irshad, A L Cookson, D J Prattley, M Dufour, N P French

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epidemiology and infection, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Shiga toxin-producingFrontiers in microbiology · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

H IrshadmEpiLab, Hopkirk ResearchInstitute,Massey University,Palmerston North,New Zealand.
A L CooksonAgResearch Ltd, Grasslands Research Centre, Palmerston North,New Zealand.
D J PrattleymEpiLab, Hopkirk ResearchInstitute,Massey University,Palmerston North,New Zealand.
M DufourEnteric Reference Laboratory, ESR-NCBID,New Zealand.
N P FrenchmEpiLab, Hopkirk ResearchInstitute,Massey University,Palmerston North,New Zealand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The objective of this study was to determine the distribution of Shiga toxin-producing Escherichia coli (STEC) virulence markers (stx1, stx2, eae, ehxA) in E. coli strains isolated from young calves aged fewer than 7 days (bobby calves). In total, 299 recto-anal mucosal swabs were collected from animals at two slaughter plants and inoculated onto tryptone bile X-glucuronide and sorbitol MacConkey agar supplemented with cefixime and potassium tellurite. Isolates were analysed using multiplex polymerase chain reaction to detect stx1, stx2, eae and ehxA genes. The most common combination of virulence markers were eae, ehxA (n = 35) followed by eae (n = 9). In total, STEC and atypical enteropathogenic E. coli (aEPEC) were isolated from 8/299 (2·6%) and 37/299 (12·3%) calves, respectively. All the isolates could be assigned to 15 genotype clusters with >70% similarity cut-off using XbaI pulsed-field gel electrophoresis. It may be concluded that healthy calves from the dairy industry are asymptomatic carriers of a diverse population of STEC and aEPEC in New Zealand.

Indexed as

AbattoirsAdhesins, BacterialAnimalsAnimals, NewbornCattleCattle DiseasesEscherichia coli ProteinsHemolysin ProteinsNew ZealandPolymerase Chain ReactionShiga-Toxigenic Escherichia coliShiga Toxin 1Shiga Toxin 2VirulenceAdhesins, BacterialeaeA protein, E coliEscherichia coli ProteinsHemolysin ProteinsHlya protein, E coliShiga Toxin 1Shiga Toxin 2

Identifiers

PMID24512720
PMCPMC9151299

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.