ArticleMolecular and cellular biology1988
Dictyostelium discoideum myosin: isolation and characterization of cDNAs encoding the essential light chain.
Article in Molecular and cellular biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 13 citations in OpenAlex.
- Thirteen is enough: the myosins of Dictyostelium discoideum and their light chains.BMC genomics · 2006Article
- The Dictyostelium class I myosin, MyoD, contains a novel light chain that lacks high-affinity calcium-binding sites.The Biochemical journal · 2003Article
- Article
- Human cardiac myosin light chains: sequence comparisons between myosin LC1 and LC2 from normal and idiopathic dilated cardiomyopathic hearts.Molecular and cellular biochemistry · 1995Article
- Specific expression of a gene encoding a novel calcium-binding protein, CAF-1, during transition of Dictyostelium cells from growth to differentiation.Development, growth & differentiation · 1995Article
- Discovery of myosin genes by physical mapping in Dictyostelium.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- The role of myosin I and II in cell motility.Cancer metastasis reviews · 1992Review
- Functional implications of the unusual amino acid sequence of the regulatory light chain of Acanthamoeba castellanii myosin-II.Journal of muscle research and cell motility · 1991Article
- Myosin light chains and troponin C: structural and evolutionary relationships revealed by amino acid sequence comparisons.Journal of muscle research and cell motility · 1991Review
- Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum.Molecular and cellular biology · 1990Article
- The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum utilizes alternate promoters and splicing for the synthesis of multiple mRNAs.Molecular and cellular biology · 1989Article
- Dictyostelium discoideum myosin: isolation and characterization of cDNAs encoding the regulatory light chain.Molecular and cellular biology · 1989Article
- Linkage analysis of the myosin heavy chain gene in Dictyostelium discoideum using a mutation generated by homologous recombination.Molecular & general genetics : MGG · 1989Article
- Regulatory myosin light-chain genes of Caenorhabditis elegans.Molecular and cellular biology · 1988Article
- Codon preference in Dictyostelium discoideum.Nucleic acids research · 1988Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used an antibody specific for Dictyostelium discoideum myosin to screen a lambda gt11 cDNA expression library to obtain cDNA clones which encode the Dictyostelium essential myosin light chain (EMLC). The amino acid sequence predicted from the sequence of the cDNA clone showed 31.5% identity with the amino acid sequence of the chicken EMLC. Comparisons of the Dictyostelium EMLC, a nonmuscle cell type, with EMLC sequences from similar MLCs of skeletal- and smooth-muscle origin, showed distinct regions of homology. Much of the observed homology was localized to regions corresponding to consensus Ca2+-binding of E-F hand domains. Southern blot analysis suggested that the Dictyostelium genome contains a single gene encoding the EMLC. Examination of the pattern of EMLC mRNA expression showed that a significant increase in EMLC message levels occurred during the first few hours of development, coinciding with increased actin expression and immediately preceding the period of maximal chemotactic activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.