ArticleCurrent protocols in cell biology2013
Probing endoplasmic reticulum dynamics using fluorescence imaging and photobleaching techniques.
Article in Current protocols in cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno-associated Virus Delivery.Current protocols · 2026Article
- Quantifying Lysosomal Degradation of Extracellular Proteins With a Fluorescent Protein-Based Internalization Assay.Bio-protocol · 2026Article
- High-speed blind structured illumination microscopy via unsupervised algorithm unrolling.Nature communications · 2026Article
- The photosensitive endoplasmic reticulum-chloroplast contact site.Journal of microscopy · 2025Article
- What determines sub-diffusive behavior in crowded protein solutions?Biophysical journal · 2024Article
- Protocol for quantification of the lysosomal degradation of extracellular proteins into mammalian cells.STAR protocols · 2021Article
- A novel automated image analysis pipeline for quantifying morphological changes to the endoplasmic reticulum in cultured human cells.BMC bioinformatics · 2021Article
- A virtuous cycle operated by ERp44 and ERGIC-53 guarantees proteostasis in the early secretory compartment.iScience · 2021Article
- Dynamic Control of Mitochondrial CaFrontiers in cell and developmental biology · 2021Article
- QuoVadoPro, an Autonomous Tool for Measuring Intracellular Dynamics using Temporal Variance.Current protocols in cell biology · 2020Article
- The Unfolded Protein Response: Detecting and Responding to Fluctuations in the Protein-Folding Capacity of the Endoplasmic Reticulum.Cold Spring Harbor perspectives in biology · 2019Review
- 3D reconstruction of endoplasmic reticulum in a hydrocarbon-secreting green alga, Botryococcus braunii (Race B).Planta · 2018Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
This unit describes approaches and tools for studying the dynamics and organization of endoplasmic reticulum (ER) membranes and proteins in living cells using fluorescence microscopy. The ER plays a key role in secretory protein biogenesis, calcium regulation, and lipid synthesis. However, study of these processes has often been restricted to biochemical assays that average millions of lysed cells or imaging of static fixed cells. With new fluorescent protein (FP) reporter tools, sensitive commercial microscopes, and photobleaching techniques, investigators can interrogate the behaviors of ER proteins, membranes, and stress pathways in single live cells. Solutions are described for imaging challenges relevant to the ER, including the mobility of ER membranes, a range of ER structures, and the influence of post-translational modifications on FP reporters. Considerations for performing photobleaching assays for ER proteins are discussed. Finally, reporters and drugs for studying misfolded secretory protein stress and the unfolded protein response are described.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.