Evidence map›Paper›PMID 24510771›Full record

ArticleCurrent protocols in cytometry2014

How to build a time-gated luminescence microscope.

Dayong Jin, Yiqing Lu, Robert C Leif, Sean Yang, Megha Rajendran, Lawrence W Miller

Abstract read
In one paragraph

Article in Current protocols in cytometry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 25 citations in OpenAlex.

  1. Development of Time-Resolved Luminescence Measurement Instruments for Biosensing and Bioimaging - An Overview.Measurement : journal of the International Measurement Confederation · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 3 countries.

Dayong JinAdvanced Cytometry Laboratories, MQ BioFocus Research Centre & Photonics Research Centre, Macquarie University, New South Wales, Australia.
Yiqing LuAdvanced Cytometry Laboratories, MQ BioFocus Research Centre & Photonics Research Centre, Macquarie University, New South Wales, Australia.
Robert C LeifNewport Instruments, San Diego, California.
Sean YangNewport Instruments, San Diego, California.
Megha RajendranDepartment of Chemistry, University of Illinois at Chicago, Chicago, Illinois.
Lawrence W MillerDepartment of Chemistry, University of Illinois at Chicago, Chicago, Illinois.
Macquarie University · AUUniversity of Illinois Chicago · USIS Instruments (United Kingdom) · GBNewport (United States) · US

Funding

Targeted lanthanide contrast agents for in cellulo single molecule imagingR01GM081030 · NIGMS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI MILLER, LAWRENCE · 2008 to 2018
$2.5M
NIGMS NIH HHS R01 GM081030NIGMS NIH HHS R01GM081030-01A1
6 · The paper itself

Abstract

The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence.

Indexed as

LuminescenceCalibrationEmulsionsMicroscopyMicrospheresOilsSignal Processing, Computer-AssistedWaterEmulsionsOilsWaterautofluorescencelanthanidemicroscopytime-gated luminescence

Identifiers

PMID24510771
PMCPMC13628505
OpenAlexW1661559393

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.