ArticlePlant physiology2014
Hydrogen peroxide acts upstream of nitric oxide in the heat shock pathway in Arabidopsis seedlings.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 144 citations in OpenAlex.
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- Genomic introgression underlies environmental adaptation in three species of Chinese wingnuts,Plant diversity · 2025Article
- Nitric oxide-mediated thermomemory: a new perspective on plant heat stress resilience.Frontiers in plant science · 2025Review
- Hydrogen peroxide sulfenylates and inhibits the photorespiratory enzyme PGLP1 to modulate plant thermotolerance.Plant communications · 2024Article
- Appraisal of the Role of Gaseous Signaling Molecules in Thermo-Tolerance Mechanisms in Plants.Plants (Basel, Switzerland) · 2024Review
- Enzymes Involved in Antioxidant and Detoxification Processes Present Changes in the Expression Levels of Their Coding Genes under the Stress Caused by the Presence of Antimony in Tomato.Plants (Basel, Switzerland) · 2024Article
- Calcium Signaling and the Response to Heat Shock in Crop Plants.International journal of molecular sciences · 2023Review
- Identification of genes associated with abiotic stress tolerance in sweetpotato using weighted gene co-expression network analysis.Plant direct · 2023Article
- Multiple Ways of Nitric Oxide Production in Plants and Its Functional Activity under Abiotic Stress Conditions.International journal of molecular sciences · 2023Review
- The Regulatory Network of Sweet Corn (International journal of molecular sciences · 2023Article
- Redox Signaling in Plant Heat Stress Response.Antioxidants (Basel, Switzerland) · 2023Review
- The Early Oxidative Stress Induced by Mercury and Cadmium Is Modulated by Ethylene inAntioxidants (Basel, Switzerland) · 2023Article
- Small Heat Shock Protein (Plants (Basel, Switzerland) · 2023Article
- Extra- and intranuclear heat perception and triggering mechanisms in plants.Frontiers in plant science · 2023Review
- The role of nitric oxide and hydrogen sulfide in regulation of redox homeostasis at extreme temperatures in plants.Frontiers in plant science · 2023Review
- Brassinosteroids promote thermotolerance through releasing BIN2-mediated phosphorylation and suppression of HsfA1 transcription factors in Arabidopsis.Plant communications · 2022Article
- Review
- Reproductive-Stage Heat Stress in Cereals: Impact, Plant Responses and Strategies for Tolerance Improvement.International journal of molecular sciences · 2022Review
- Crosstalk between Melatonin and Reactive Oxygen Species in Plant Abiotic Stress Responses: An Update.International journal of molecular sciences · 2022Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reported that nitric oxide (NO) functions as a signal in thermotolerance. To illustrate its relationship with hydrogen peroxide (H₂O₂) in the tolerance of Arabidopsis (Arabidopsis thaliana) to heat shock (HS), we investigated the effects of heat on Arabidopsis seedlings of the following types: the wild type; three NADPH oxidase-defective mutants that exhibit reduced endogenous H₂O₂ levels (atrbohB, atrbohD, and atrbohB/D); and a mutant that is resistant to inhibition by fosmidomycin (noa1, for nitric oxide-associated protein1). After HS, the NO levels in atrbohB, atrbohD, and atrbohB/D seedlings were lower than that in wild-type seedlings. Treatment of the seedlings with sodium nitroprusside or S-nitroso-N-acetylpenicillamine partially rescued their heat sensitivity, suggesting that NO is involved in H₂O₂ signaling as a downstream factor. This point was verified by phenotypic analyses and thermotolerance testing of transgenic seedlings that overexpressed Nitrate reductase2 and NOA1, respectively, in an atrbohB/D background. Electrophoretic mobility shift assays, western blotting, and real-time reverse transcription-polymerase chain reaction demonstrated that NO stimulated the DNA-binding activity of HS factors and the accumulation of HS proteins through H₂O₂. These data indicate that H₂O₂ acts upstream of NO in thermotolerance, which requires increased HS factor DNA-binding activity and HS protein accumulation.
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