Evidence map›Paper›PMID 24510648›Full record

ArticleCurrent protocols in protein science2013

Methods for studying interactions of detergents and lipids with α-helical and β-barrel integral membrane proteins.

S Saif Hasan, Danas Baniulis, Eiki Yamashita, Mariya V Zhalnina, Stanislav D Zakharov, Jason T Stofleth, William A Cramer

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current protocols in protein science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 4 countries.

S Saif HasanDepartment of Biological Sciences, Purdue University, West Lafayette, Indiana.
Danas BaniulisDepartment of Biological Sciences, Purdue University, West Lafayette, Indiana.
Eiki YamashitaInstitute for Protein Research, Osaka University, Suita, Osaka, Japan.
Mariya V ZhalninaDepartment of Biological Sciences, Purdue University, West Lafayette, Indiana.
Stanislav D ZakharovDepartment of Biological Sciences, Purdue University, West Lafayette, Indiana.
Jason T StoflethDepartment of Biological Sciences, Purdue University, West Lafayette, Indiana.
William A CramerDepartment of Biological Sciences, Purdue University, West Lafayette, Indiana.
Purdue University West Lafayette · USLithuanian Research Centre for Agriculture and Forestry · LTOsaka University · JPUniversity of California, San Diego · US

Funding

Trans-Membrane Signaling in the Cytochrome b6f Lipoprotein ComplexR01GM038323 · NIGMS · PURDUE UNIVERSITY WEST LAFAYETTE · PI CRAMER, WILLIAM A. · 1987 to 2017
$5.1M
THE MECHANISM OF ACTION OF COLICIN E1R01GM018457 · NIGMS · PURDUE UNIVERSITY WEST LAFAYETTE · PI CRAMER, WILLIAM A. · 1985 to 2007
$2.2M
Structure-Function of Hetero-oligomeric Integral Membrane ProteinsR56GM038323 · NIGMS · PURDUE UNIVERSITY · PI CRAMER, WILLIAM A. · 2007 to 2007
$336k
NIGMS NIH HHS GM-018457NIGMS NIH HHS GM-038323NIGMS NIH HHS R01 GM018457NIGMS NIH HHS R01 GM038323
6 · The paper itself

Abstract

Methods for studying interactions of protein with lipids and detergents are described for representatives of two major classes of membrane proteins: (1) the α-helical hetero-oligomeric integral cytochrome b6 f complex of oxygenic photosynthesis from cyanobacteria, and (2) the outer membrane β-barrel proteins BtuB and OmpF from Gram-negative Escherichia coli bacteria. Details are presented on the use of detergents for purification and crystallization of the b6 f complex as well as a method for lipid exchange. The positions of detergent and lipid molecules, which define eight potential lipid-binding sites in the b6 f complex, are described. Differences in detergent strategies for isolation and crystallization of β-barrel proteins relative to those for oligomeric helical membrane proteins are discussed, and purification and assessment of protein quality by circular dichroism (CD) is presented.

Indexed as

Bacterial Outer Membrane ProteinsChromatography, AffinityCircular DichroismCrystallizationCytochrome b6f ComplexDetergentsEscherichia coli ProteinsLipidsMembrane ProteinsMembrane Transport ProteinsModels, MolecularPorinsProtein Structure, SecondaryBacterial Outer Membrane ProteinsBtuB protein, E coliCytochrome b6f ComplexDetergentsEscherichia coli ProteinsLipidsMembrane ProteinsMembrane Transport ProteinsOmpF proteinPorinsBtuBcytochrome b6f complexOmpF porinvitamin B12 receptor

Identifiers

PMID24510648
PMCPMC4062877
OpenAlexW1874627940

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.