ArticleNature chemical biology2014
Ubiquinone accumulation improves osmotic-stress tolerance in Escherichia coli.
Article in Nature chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
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- Coenzyme Q10 Enhances Resilience of Mitochondrial-like Membranes Against Amyloidogenic Peptides.Membranes · 2025Article
- Low functional change despite high taxonomic turnover characterizes theScience advances · 2025Article
- Methanotroph-methylotroph lipid adaptations to changing environmental conditions.Frontiers in microbiology · 2025Article
- Modulation of Biological Membranes Using Small-Molecule Compounds to Counter Toxicity Caused by Amyloidogenic Proteins.Membranes · 2024Review
- Understanding coenzyme Q.Physiological reviews · 2024Review
- Cancer cell stiffening via CoQNature communications · 2024Article
- Advances in Integrated Multi-omics Analysis for Drug-Target Identification.Biomolecules · 2024Review
- Physiological adventures inMicrobiology and molecular biology reviews : MMBR · 2024Review
- Bacterial lipid biophysics and membrane organization.Current opinion in microbiology · 2023Review
- Carotenoids synthesis affects the salt tolerance mechanism ofFrontiers in microbiology · 2023Article
- Evolutionary action of mutations reveals antimicrobial resistance genes in Escherichia coli.Nature communications · 2022Article
- Mediator Engineering ofApplied and environmental microbiology · 2022Article
- Antibiotic tolerance in environmentally stressedmicroLife · 2022Article
- Effect of low power lasers on prokaryotic and eukaryotic cells under different stress condition: a review of the literature.Lasers in medical science · 2021Review
- Location and Conformational Ensemble of Menaquinone and Menaquinol, and Protein-Lipid Modulations in Archaeal Membranes.The journal of physical chemistry. B · 2021Article
- Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens.Scientific reports · 2021Article
- Global coordination of metabolic pathways in Escherichia coli by active and passive regulation.Molecular systems biology · 2021Article
- Native-like membrane models of E. coli polar lipid extract shed light on the importance of lipid composition complexity.BMC biology · 2021Article
4 more citing papers are in PubMed but not listed here.
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Bacteria are thought to cope with fluctuating environmental solute concentrations primarily by adjusting the osmolality of their cytoplasm. To obtain insights into the underlying metabolic adaptations, we analyzed the global metabolic response of Escherichia coli to sustained hyperosmotic stress using nontargeted mass spectrometry. We observed that 52% of 1,071 detected metabolites, including known osmoprotectants, changed abundance with increasing salt challenge. Unexpectedly, unsupervised data analysis showed a substantial increase of most intermediates in the ubiquinone-8 (Q8) biosynthesis pathway and a 110-fold accumulation of Q8 itself, as confirmed by quantitative lipidomics. We then demonstrated that Q8 is necessary for acute and sustained osmotic-stress tolerance using Q8 mutants and tolerance rescue through feeding nonrespiratory Q8 analogs. Finally, in vitro experiments with artificial liposomes showed that mechanical membrane stabilization is a principal mechanism of Q8-mediated osmoprotection. Thus, we find that besides regulating intracellular osmolality, E. coli enhances its cytoplasmic membrane stability to withstand osmotic stress.
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