ArticleJournal of bacteriology2014
Identification of an O-acyltransferase gene (oacB) that mediates 3- and 4-O-acetylation of rhamnose III in Shigella flexneri O antigens.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- O-antigen polysaccharides inMicrobiology and molecular biology reviews : MMBR · 2025Review
- Bacteriophage Sf6 host range mutant that infects Shigella flexneri serotype 2a2 strains.FEMS microbiology letters · 2022Article
- Identification of critical residues of O-antigen-modifying O-acetyltransferase B (OacB) of Shigella flexneri.BMC molecular and cell biology · 2022Article
- Diverse functions for acyltransferase-3 proteins in the modification of bacterial cell surfaces.Microbiology (Reading, England) · 2022Article
- Impact of O-Acetylation onMicroorganisms · 2021Article
- Influence of Shigella flexneri 2a O Antigen Acetylation on Its Bacteriophage Sf6 Receptor Activity and Bacterial Interaction with Human Cells.Journal of bacteriology · 2020Article
- Article
- PCR-Based Method for Shigella flexneri Serotyping: International Multicenter Validation.Journal of clinical microbiology · 2019Article
- Bacteriophage Sf6 Tailspike Protein for Detection ofViruses · 2018Article
- Bacteriophages are the major drivers of Shigella flexneri serotype 1c genome plasticity: a complete genome analysis.BMC genomics · 2017Article
- WciGJournal of clinical microbiology · 2017Article
- Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrIC gene cluster via a bacteriophage.BMC microbiology · 2016Article
- Genetic and structural identification of an O-acyltransferase gene (oacC) responsible for the 3/4-O-acetylation on rhamnose III in Shigella flexneri serotype 6.BMC microbiology · 2014Article
- Serotype-converting bacteriophage SfII encodes an acyltransferase protein that mediates 6-O-acetylation of GlcNAc in Shigella flexneri O-antigens, conferring on the host a novel O-antigen epitope.Journal of bacteriology · 2014Article
- Serotype-conversion in Shigella flexneri: identification of a novel bacteriophage, Sf101, from a serotype 7a strain.BMC genomics · 2014Article
- Serological identification and prevalence of a novel O-antigen epitope linked to 3- and 4-O-acetylated rhamnose III of lipopolysaccharide in Shigella flexneri.Journal of clinical microbiology · 2014Article
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
O antigen (O polysaccharide) is an important and highly variable cell component present on the surface of cells which defines the serospecificity of Gram-negative bacteria. Most O antigens of Shigella flexneri, a cause of shigellosis, share a backbone composed of →2)-α-l-Rhap(III)-(1→2)-α-l-Rhap(II)-(1→3)-α-l-Rhap(I)-(1→3)-β-d-GlcpNAc-(1→ repeats, which can be modified by adding various substituents, giving rise to 19 serotypes. The known modifications include glucosylation on various sugar residues, O-acetylation on Rha(I), and phosphorylation with phosphoethanolamine on Rha(II) or/and Rha(III). Recently, two new O-antigen modifications, namely, O-acetylation at position 3 or 4 of Rha(III) and position 6 of GlcNAc, have been identified in several S. flexneri serotypes. In this work, the genetic basis for the 3/4-O-acetylation on Rha(III) was elucidated. Bioinformatic analysis of the genome of S. flexneri serotype 2a strain Sf301, which carries 3/4-O-acetylation on Rha(III), revealed an O-acyltransferase gene designated oacB. Genetic studies combined with O-antigen structure analysis demonstrated that this gene is responsible for the 3/4-O-acetylation in serotypes 1a, 1b, 2a, 5a, and Y but not serotype 6, which has a different O-antigen backbone structure. The oacB gene is carried by a transposon-like structure located in the proA-adrA region on the chromosome, which represents a novel mechanism of mobilization of O-antigen modification factors in S. flexneri. These findings enhance our knowledge of S. flexneri O-antigen modifications and shed light on the origin of new O-antigen variants.
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