ArticleAnnals of surgery2014
Comparison of cancer-associated genetic abnormalities in columnar-lined esophagus tissues with and without goblet cells.
Article in Annals of surgery, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- ACG Clinical Guideline: Diagnosis and Management of Barrett's Esophagus.The American journal of gastroenterology · 2016Guideline
- Article
- Article
- Tumor cell malignancy: A complex trait built through reciprocal interactions between tumors and tissue-body system.iScience · 2022Review
- DNA methylation markers in esophageal cancer: an emerging tool for cancer surveillance and treatment.American journal of cancer research · 2021Review
- Barrett's Esophagus and Intestinal Metaplasia.Frontiers in oncology · 2021Review
- Gastro-esophageal reflux disease and Barrett's esophagus: an overview with an histologic diagnostic approach.Pathologica · 2020Review
- Reply to Letter Regarding "Barrett's esophagus and Sleeve Gastrectomy".Obesity surgery · 2019Article
- Prevalence and risk factors for Barrett's esophagus in Taiwan.World journal of gastroenterology · 2019Article
- Histology of Barrett's Metaplasia: Do Goblet Cells Matter?Digestive diseases and sciences · 2018Review
- Is Barrett's-Associated Esophageal Adenocarcinoma a Clonal Disease?Digestive diseases and sciences · 2018Review
- Current Concepts in Treatment of Barrett's Esophagus With and Without Dysplasia.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2017Review
- Barrett's oesophagus: Current controversies.World journal of gastroenterology · 2017Review
- Past, present and future of Barrett's oesophagus.European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology · 2017Review
- From Heartburn to Barrett's Esophagus, and Beyond.World journal of surgery · 2017Review
- Optimizing the diagnosis and therapy of Barrett's esophagus.Journal of thoracic disease · 2017Review
- The characterization of an intestine-like genomic signature maintained during Barrett's-associated adenocarcinogenesis reveals an NR5A2-mediated promotion of cancer cell survival.Scientific reports · 2016Article
- Article
- Barrett's Esophagus: A Comprehensive and Contemporary Review for Pathologists.The American journal of surgical pathology · 2016Review
- Barrett's oesophagus: frequency and prediction of dysplasia and cancer.Best practice & research. Clinical gastroenterology · 2015Review
Corrections and comments
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Authors and funding
20 authors at 7 institutions in 2 countries.
Funding
Abstract
objectiveTo determine and compare the frequency of cancer-associated genetic abnormalities in esophageal metaplasia biopsies with and without goblet cells.
backgroundBarrett's esophagus is associated with increased risk of esophageal adenocarcinoma (EAC), but the appropriate histologic definition of Barrett's esophagus is debated. Intestinal metaplasia (IM) is defined by the presence of goblet cells whereas nongoblet cell metaplasia (NGM) lacks goblet cells. Both have been implicated in EAC risk but this is controversial. Although IM is known to harbor genetic changes associated with EAC, little is known about NGM. We hypothesized that if NGM and IM infer similar EAC risk, then they would harbor similar genetic aberrations in genes associated with EAC.
methodsNinety frozen NGM, IM, and normal tissues from 45 subjects were studied. DNA copy number abnormalities were identified using microarrays and fluorescence in situ hybridization. Targeted sequencing of all exons from 20 EAC-associated genes was performed on metaplasia biopsies using Ion AmpliSeq DNA sequencing.
resultsFrequent copy number abnormalities targeting cancer-associated genes were found in IM whereas no such changes were observed in NGM. In 1 subject, fluorescence in situ hybridization confirmed loss of CDKN2A and amplification of chromosome 8 in IM but not in a nearby NGM biopsy. Targeted sequencing revealed 11 nonsynonymous mutations in 16 IM samples and 2 mutations in 19 NGM samples.
conclusionsThis study reports the largest and most comprehensive comparison of DNA aberrations in IM and NGM genomes. Our results show that IM has a much higher frequency of cancer-associated mutations than NGM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.