Evidence map›Paper›PMID 24508590›Full record

ArticleProtein expression and purification2014

A semi-automated method for purification of milligram quantities of proteins on the QIAcube.

J McGraw, V K Tatipelli, O Feyijinmi, M C Traore, P Eangoor, S Lane, E J Stollar

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein expression and purification, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Automation in the Life Science Research Laboratory.Frontiers in bioengineering and biotechnology · 2020
    Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

J McGrawEastern New Mexico University, Department of Physical Sciences, Station #33, Portales, NM 88130, United States.
V K TatipelliEastern New Mexico University, Department of Physical Sciences, Station #33, Portales, NM 88130, United States.
O FeyijinmiEastern New Mexico University, Department of Physical Sciences, Station #33, Portales, NM 88130, United States.
M C TraoreEastern New Mexico University, Department of Physical Sciences, Station #33, Portales, NM 88130, United States.
P EangoorEastern New Mexico University, Department of Physical Sciences, Station #33, Portales, NM 88130, United States.
S LaneEastern New Mexico University, Department of Physical Sciences, Station #33, Portales, NM 88130, United States.
E J StollarEastern New Mexico University, Department of Physical Sciences, Station #33, Portales, NM 88130, United States. Electronic address: elliott.stollar@enmu.edu.

Funding

NM-INBRE Sequencing and Bioinformatics CoreP20GM103451 · NIGMS · NEW MEXICO STATE UNIVERSITY LAS CRUCES · PI Charlotte C. Gard · 2012 to 2026
$61.2M
ZUNI HEALTH INITIATIVE-PILOT STUDYP20RR016480 · NCRR · NEW MEXICO STATE UNIVERSITY LAS CRUCES · PI ZHANG, PENG F · 2001 to 2011
$33.7M
NCRR NIH HHS 5P20RR016480-12NCRR NIH HHS P20 RR016480NIGMS NIH HHS 8P20GM103451-12NIGMS NIH HHS P20 GM103451
6 · The paper itself

Abstract

A growing number of studies require the purification of multiple proteins simultaneously and the development of simple economical high-throughput purification methods is essential. We have tested the purification of two related proteins in a variety of conditions to benchmark the semi-automated affinity chromatography method for the QIAcube that we have developed. We find that this new QIAcube method can successfully purify milligram quantities of proteins with minimal user involvement and performs as well as methods based on gravity. The method could easily be adapted to other chromatography resins and should prove to be a versatile method for optimizing protein expression or purification conditions for multiple proteins while obtaining sufficient amounts for subsequent biochemical analyses.

Indexed as

CentrifugationChromatography, AffinityGene ExpressionHigh-Throughput Screening AssaysProtein BiosynthesisProteinsProteomicsProteinsHigh-throughput automationProtein expressionProtein purificationQIAcubeSH3 domain

Identifiers

PMID24508590
PMCPMC4287359

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.