ArticleCurrent biology : CB2014
Dueling kinases regulate cell size at division through the SAD kinase Cdr2.
Article in Current biology : CB, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division.The Journal of cell biology · 2022Article
- Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1.Molecular biology of the cell · 2021Article
- Phosphoinositide-Dependent Protein Kinases Regulate Cell Cycle Progression Through the SAD Kinase Cdr2 in Fission Yeast.Frontiers in microbiology · 2021Article
- The phosphatase inhibitor Sds23 promotes symmetric spindle positioning in fission yeast.Cytoskeleton (Hoboken, N.J.) · 2020Article
- Phosphoregulation of tropomyosin is crucial for actin cable turnover and division site placement.The Journal of cell biology · 2019Article
- Growth and the cell cycle in green algae dividing by multiple fission.Folia microbiologica · 2019Review
- A new identified suppressor of Cdc7p/SepH kinase, PomA, regulates fungal asexual reproduction via affecting phosphorylation of MAPK-HogA.PLoS genetics · 2019Article
- Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size.eLife · 2019Article
- Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry.eLife · 2019Article
- Reprogramming Cdr2-Dependent Geometry-Based Cell Size Control in Fission Yeast.Current biology : CB · 2019Article
- Cell size-dependent regulation of Wee1 localization by Cdr2 cortical nodes.The Journal of cell biology · 2018Article
- Dynamic visits of cortical structures probe for cell size.The Journal of cell biology · 2018Article
- Mechanisms Connecting the Conserved Protein Kinases Ssp1, Kin1, and Pom1 in Fission Yeast Cell Polarity and Division.Current biology : CB · 2018Article
- Controlling cell size through sizer mechanisms.Current opinion in systems biology · 2017Review
- Transient activation of fission yeast AMPK is required for cell proliferation during osmotic stress.Molecular biology of the cell · 2017Article
- Phosphatases Generate Signal Specificity Downstream of Ssp1 Kinase in Fission Yeast.Molecular and cellular biology · 2017Article
- A new class of cyclin dependent kinase in Chlamydomonas is required for coupling cell size to cell division.eLife · 2016Article
- The DYRK-family kinase Pom1 phosphorylates the F-BAR protein Cdc15 to prevent division at cell poles.The Journal of cell biology · 2015Article
- PKA antagonizes CLASP-dependent microtubule stabilization to re-localize Pom1 and buffer cell size upon glucose limitation.Nature communications · 2015Article
- Pom1 gradient buffering through intermolecular auto-phosphorylation.Molecular systems biology · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Cell size control requires mechanisms that integrate cell growth and division. Key to this integration in fission yeast is the SAD family kinase Cdr2, which organizes a set of cortical nodes in the cell middle to promote mitotic entry through Wee1 and Cdk1. Cdr2 is inhibited by a spatial gradient of the DYRK kinase Pom1 emanating from cell tips in a cell-size-dependent manner, but how the Pom1 gradient inhibits Cdr2 activity during cell growth is unknown. Here, we show that Pom1 acts to prevent activation of Cdr2 kinase activity by the CaMKK Ssp1. We found that Ssp1 activates Cdr2 through phosphorylation of a conserved threonine residue (Thr166) in the activation loop of the Cdr2 N-terminal kinase domain both in vitro and in cells. The levels of this activating phosphorylation increased with cell-cycle progression, and genetic epistasis demonstrated that Ssp1 promotes mitotic entry through Cdr2. Intriguingly, Pom1 phosophorylated the C-terminal domain of Cdr2, and this modification reduced Cdr2-T166 phosphorylation by Ssp1. These findings show how activation of the conserved mitotic inducer Cdr2 is integrated with an inhibitory spatial gradient to ensure proper cell size control at mitosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.