ArticleBiomaterials2014
Robust T-tubulation and maturation of cardiomyocytes using tissue-engineered epicardial mimetics.
Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Substrate mechanics unveil early structural and functional pathology in iPSC micro-tissue models of hypertrophic cardiomyopathy.iScience · 2024Article
- Selective Induction of Molecular Assembly to Tissue-Level Anisotropy on Peptide-Based Optoelectronic Cardiac Biointerfaces.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Heart-on-a-chip systems with tissue-specific functionalities for physiological, pathological, and pharmacological studies.Materials today. Bio · 2024Review
- Leaf-venation-directed cellular alignment for macroscale cardiac constructs with tissue-like functionalities.Nature communications · 2023Article
- How to fix a broken heart-designing biofunctional cues for effective, environmentally-friendly cardiac tissue engineering.Frontiers in chemistry · 2023Review
- Human-induced pluripotent stem cell-atrial-specific cardiomyocytes and atrial fibrillation.World journal of clinical cases · 2022Review
- Progress in Bioengineering Strategies for Heart Regenerative Medicine.International journal of molecular sciences · 2022Review
- Engineering of thick human functional myocardium via static stretching and electrical stimulation.iScience · 2022Article
- CRISPR Library Screening in Cultured Cardiomyocytes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Extracellular Vesicles in Cardiac Regeneration: Potential Applications for Tissues-on-a-Chip.Trends in biotechnology · 2021Review
- Cardiac mechanostructure: Using mechanics and anisotropy as inspiration for developing epicardial therapies in treating myocardial infarction.Bioactive materials · 2021Review
- Engineering aligned human cardiac muscle using developmentally inspired fibronectin micropatterns.Scientific reports · 2021Article
- Next generation of heart regenerative therapies: progress and promise of cardiac tissue engineering.NPJ Regenerative medicine · 2021Review
- Mechanical stimulation enhances development of scaffold-free, 3D-printed, engineered heart tissue grafts.Journal of tissue engineering and regenerative medicine · 2021Article
- Physiological and pharmacological stimulation for in vitro maturation of substrate metabolism in human induced pluripotent stem cell-derived cardiomyocytes.Scientific reports · 2021Article
- Recapitulating Cardiac Structure and Function In Vitro from Simple to Complex Engineering.Micromachines · 2021Review
- Engineering Cardiovascular Tissue Chips for Disease Modeling and Drug Screening Applications.Frontiers in bioengineering and biotechnology · 2021Review
- Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues.RSC advances · 2020Article
- Long-term contractile activity and thyroid hormone supplementation produce engineered rat myocardium with adult-like structure and function.Acta biomaterialia · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Complex three-dimensional (3-D) heart structure is an important determinant of cardiac electrical and mechanical function. In this study, we set to develop a versatile tissue-engineered system that can promote important aspects of cardiac functional maturation and reproduce variations in myofiber directions present in native ventricular epicardium. We cultured neonatal rat cardiomyocytes within a 3-D hydrogel environment using microfabricated elastomeric molds with hexagonal posts. By varying individual post orientations along the directions derived from diffusion tensor magnetic resonance imaging (DTMRI) maps of human ventricle, we created large (2.5 × 2.5 cm(2)) 3-D cardiac tissue patches with cardiomyocyte alignment that replicated human epicardial fiber orientations. After 3 weeks of culture, the advanced structural and functional maturation of the engineered 3-D cardiac tissues compared to age-matched 2-D monolayers was evident from: 1) the presence of dense, aligned and electromechanically-coupled cardiomyocytes, quiescent fibroblasts, and interspersed capillary-like structures, 2) action potential propagation with near-adult conduction velocity and directional dependence on local cardiomyocyte orientation, and 3) robust formation of T-tubules aligned with Z-disks, co-localization of L-type Ca(2+) channels and ryanodine receptors, and accelerated Ca(2+) transient kinetics. This biomimetic tissue-engineered platform can enable systematic in vitro studies of cardiac structure-function relationships and promote the development of advanced tissue engineering strategies for cardiac repair and regeneration.
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