ArticleBiophysical journal2014
Temporal dynamics of microbial rhodopsin fluorescence reports absolute membrane voltage.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Voltage imaging: A practical guide.Neuron · 2026Review
- Absolute voltage mapping using dynamic photocycle control.bioRxiv : the preprint server for biology · 2026Article
- Absolute Membrane Potential Recording with ASAP-Type Genetically Encoded Voltage Indicators Using Fluorescence Lifetime Imaging.ACS chemical neuroscience · 2025Article
- Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators.Science advances · 2025Article
- A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic events.Neuron · 2024Article
- Nitrile Vibrational Lifetimes as Probes of Local Electric Fields.The journal of physical chemistry letters · 2024Article
- An Ultrasensitive Genetically Encoded Voltage Indicator Uncovers the Electrical Activity of Non-Excitable Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators.bioRxiv : the preprint server for biology · 2024Article
- Fluorescence Imaging of Cell Membrane Potential: From Relative Changes to Absolute Values.International journal of molecular sciences · 2023Review
- Monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators.Communications biology · 2021Article
- Advanced Optogenetic-Based Biosensing and Related Biomaterials.Materials (Basel, Switzerland) · 2021Review
- Measuring Absolute Membrane Potential Across Space and Time.Annual review of biophysics · 2021Review
- Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter.Science advances · 2021Article
- High-speed compressed-sensing fluorescence lifetime imaging microscopy of live cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Imaging Voltage with Microbial Rhodopsins.Frontiers in molecular biosciences · 2021Review
- Interrogating Cellular Communication in Cancer with Genetically Encoded Imaging Reporters.Radiology. Imaging cancer · 2020Review
- Article
- Red Fluorescent Genetically Encoded Voltage Indicators with Millisecond Responsiveness.Sensors (Basel, Switzerland) · 2019Article
- Simultaneous voltage and calcium imaging and optogenetic stimulation with high sensitivity and a wide field of view.Biomedical optics express · 2019Article
- Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming.Journal of the American Chemical Society · 2019Article
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3 authors.
Funding
Abstract
Plasma membrane voltage is a fundamentally important property of a living cell; its value is tightly coupled to membrane transport, the dynamics of transmembrane proteins, and to intercellular communication. Accurate measurement of the membrane voltage could elucidate subtle changes in cellular physiology, but existing genetically encoded fluorescent voltage reporters are better at reporting relative changes than absolute numbers. We developed an Archaerhodopsin-based fluorescent voltage sensor whose time-domain response to a stepwise change in illumination encodes the absolute membrane voltage. We validated this sensor in human embryonic kidney cells. Measurements were robust to variation in imaging parameters and in gene expression levels, and reported voltage with an absolute accuracy of 10 mV. With further improvements in membrane trafficking and signal amplitude, time-domain encoding of absolute voltage could be applied to investigate many important and previously intractable bioelectric phenomena.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.