Evidence map›Paper›PMID 24507565›Full record

ArticleBioelectrochemistry (Amsterdam, Netherlands)2014

Disassembly of actin structures by nanosecond pulsed electric field is a downstream effect of cell swelling.

Andrei G Pakhomov, Shu Xiao, Olga N Pakhomova, Iurii Semenov, Marjorie A Kuipers, Bennett L Ibey

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioelectrochemistry (Amsterdam, Netherlands), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
9.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 74 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Andrei G PakhomovFrank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA. Electronic address: 2andrei@pakhomov.net.
Shu XiaoFrank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA; Dept. of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA, USA.
Olga N PakhomovaFrank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA.
Iurii SemenovFrank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA.
Marjorie A KuipersRadio Frequency Bioeffects Branch, Bioeffects Division, Human Effectiveness Directorate, 711th Human Performance Wing, Air Force Research Laboratory, Fort Sam Houston, TX, USA.
Bennett L IbeyRadio Frequency Bioeffects Branch, Bioeffects Division, Human Effectiveness Directorate, 711th Human Performance Wing, Air Force Research Laboratory, Fort Sam Houston, TX, USA.
Old Dominion University · USUnited States Air Force Research Laboratory · US

Funding

Mechanisms and Implications of Nanoelectroporation in Living CellsR01GM088303 · NIGMS · OLD DOMINION UNIVERSITY · PI PAKHOMOV, ANDREI G · 2010 to 2013
$1.1M
Cell Death Induction by High-Voltage, Nanosecond-duration Electric PulsesR01CA125482 · NCI · OLD DOMINION UNIVERSITY · PI PAKHOMOV, ANDREI G · 2008 to 2011
$1.1M
NCI NIH HHS R01 CA125482NCI NIH HHS R01CA125482NIGMS NIH HHS R01 GM088303NIGMS NIH HHS R01GM088303
6 · The paper itself

Abstract

Disruption of the actin cytoskeleton structures was reported as one of the characteristic effects of nanosecond-duration pulsed electric field (nsPEF) in both mammalian and plant cells. We utilized CHO cells that expressed the monomeric fluorescent protein (mApple) tagged to actin to test if nsPEF modifies the cell actin directly or as a consequence of cell membrane permeabilization. A train of four 600-ns pulses at 19.2 kV/cm (2 Hz) caused immediate cell membrane poration manifested by YO-PRO-1 dye uptake, gradual cell rounding and swelling. Concurrently, bright actin features were replaced by dimmer and uniform fluorescence of diffuse actin. To block the nsPEF-induced swelling, the bath buffer was isoosmotically supplemented with an electropore-impermeable solute (sucrose). A similar addition of a smaller, electropore-permeable solute (adonitol) served as a control. We demonstrated that sucrose efficiently blocked disassembly of actin features by nsPEF, whereas adonitol did not. Sucrose also attenuated bleaching of mApple-tagged actin in nsPEF-treated cells (as integrated over the cell volume), although did not fully prevent it. We conclude that disintegration of the actin cytoskeleton was a result of cell swelling, which, in turn, was caused by cell permeabilization by nsPEF and transmembrane diffusion of solutes which led to the osmotic imbalance.

Indexed as

ElectricityElectroporationActinsAnimalsCell ShapeCell SizeCHO CellsCricetinaeCricetulusCytoskeletonOsmosisActinsCytoskeletonElectric pulsesElectroporationNanoporesPlasma membrane

Identifiers

PMID24507565
PMCPMC4105330
OpenAlexW2052639082

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.