ArticleThe journal of physical chemistry. B2014
Measurement and control of pH in the aqueous interior of reverse micelles.
Article in The journal of physical chemistry. B, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- Role of Acids in Stabilizing Reverse Micelles: Insights from Dodecyl Sulfate.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Rapidly Screening the Correlation between the Rotational Mobility and the Hydrogen Bonding Strength of Confined Water.The journal of physical chemistry. B · 2024Article
- Characterization of 10MAG/LDAO reverse micelles: Understanding versatility for protein encapsulation.Biophysical chemistry · 2024Article
- Investigating protein-membrane interactions using native reverse micelles constructed from naturally sourced lipids.Protein science : a publication of the Protein Society · 2023Article
- Optimization of Biocompatibility for a Hydrophilic Biological Molecule Encapsulation System.Molecules (Basel, Switzerland) · 2022Article
- The Interfacial Interactions of Glycine and Short Glycine Peptides in Model Membrane Systems.International journal of molecular sciences · 2020Article
- Slow Proton Transfer in Nanoconfined Water.ACS central science · 2020Article
- Extending the Detection Limit in Fragment Screening of Proteins Using Reverse Micelle Encapsulation.ACS chemical biology · 2019Article
- Site-Resolved and Quantitative Characterization of Very Weak Protein-Ligand Interactions.ACS chemical biology · 2019Article
- Reverse Micelle Encapsulation of Proteins for NMR Spectroscopy.Methods in enzymology · 2019Article
- Characterizing Protein Hydration Dynamics Using Solution NMR Spectroscopy.Methods in enzymology · 2019Article
- Modulation of the Excited-State Proton Transfer Rate of d-luciferin in Mixed Reverse Micellar Systems.ACS omega · 2018Article
- Article
- High-resolution NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Review
- Optimized reverse micelle surfactant system for high-resolution NMR spectroscopy of encapsulated proteins and nucleic acids dissolved in low viscosity fluids.Journal of the American Chemical Society · 2014Article
- Reverse micelles as a platform for dynamic nuclear polarization in solution NMR of proteins.Journal of the American Chemical Society · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The encapsulation of proteins and nucleic acids within the nanoscale water core of reverse micelles has been used for over 3 decades as a vehicle for a wide range of investigations including enzymology, the physical chemistry of confined spaces, protein and nucleic acid structural biology, and drug development and delivery. Unfortunately, the static and dynamical aspects of the distribution of water in solutions of reverse micelles complicate the measurement and interpretation of fundamental parameters such as pH. This is a severe disadvantage in the context of (bio)chemical reactions and protein structure and function, which are generally highly sensitive to pH. There is a need to more fully characterize and control the effective pH of the reverse micelle water core. The buffering effect of titratable head groups of the reverse micelle surfactants is found to often be the dominant variable defining the pH of the water core. Methods for measuring the pH of the reverse micelle aqueous interior using one-dimensional (1)H and two-dimensional heteronuclear NMR spectroscopy are described. Strategies for setting the effective pH of the reverse micelle water core are demonstrated. The exquisite sensitivity of encapsulated proteins to the surfactant, water content, and pH of the reverse micelle is also addressed. These results highlight the importance of assessing the structural fidelity of the encapsulated protein using multidimensional NMR before embarking upon a detailed structural and biophysical characterization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.