ArticleTraffic (Copenhagen, Denmark)2014
Ultrafast diffusion of a fluorescent cholesterol analog in compartmentalized plasma membranes.
Article in Traffic (Copenhagen, Denmark), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 79 citations in OpenAlex.
- Using DNA Origami to Study Nanoscale Organization of Plasma Membranes.Nano letters · 2026Article
- Five key challenges for the plasma membrane insertion of nicotinic acetylcholine receptors in cellular model systems.Molecular and cellular biochemistry · 2026Review
- Development of ultrafast single fluorescent-molecule imaging and its application to unravel plasma membrane structure and function in live cells.Biophysics and physicobiology · 2026Article
- Review
- Concurrent diffusion of nicotinic acetylcholine receptors and fluorescent cholesterol disclosed by two-colour sub-millisecond MINFLUX-based single-molecule tracking.Nature communications · 2025Article
- Quantifying biomolecular organisation in membranes with brightness-transit statistics.Nature communications · 2024Article
- A non-toxic equinatoxin-II reveals the dynamics and distribution of sphingomyelin in the cytosolic leaflet of the plasma membrane.Scientific reports · 2024Article
- High-speed optical imaging with sCMOS pixel reassignment.Nature communications · 2024Article
- Sub-membrane actin rings compartmentalize the plasma membrane.The Journal of cell biology · 2024Article
- Dynamic Mode Decomposition of Multiphoton and Stimulated Emission Depletion Microscopy Data for Analysis of Fluorescent Probes in Cellular Membranes.Sensors (Basel, Switzerland) · 2024Article
- Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging.Journal of imaging · 2023Review
- Development of ultrafast camera-based single fluorescent-molecule imaging for cell biology.The Journal of cell biology · 2023Article
- Development of lacto-series ganglioside fluorescent probe using late-stage sialylation and behavior analysis with single-molecule imaging.RSC chemical biology · 2022Article
- Imaging Membrane Order and Dynamic Interactions in Living Cells with a DNA Zipper Probe.Angewandte Chemie (International ed. in English) · 2022Article
- Determination of the size of lipid rafts studied through single-molecule FRET simulations.Biophysical journal · 2021Article
- Cholesterol stabilizes recombinant exocytic fusion pores by altering membrane bending rigidity.Biophysical journal · 2021Article
- High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases.The Journal of cell biology · 2020Article
- Analyzing protein dynamics from fluorescence intensity traces using unsupervised deep learning network.Communications biology · 2020Article
- Dynamic actin-mediated nano-scale clustering of CD44 regulates its meso-scale organization at the plasma membrane.Molecular biology of the cell · 2020Article
- Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells.Frontiers in immunology · 2020Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cholesterol distribution and dynamics in the plasma membrane (PM) are poorly understood. The recent development of Bodipy488-conjugated cholesterol molecule (Bdp-Chol) allowed us to study cholesterol behavior in the PM, using single fluorescent-molecule imaging. Surprisingly, in the intact PM, Bdp-Chol diffused at the fastest rate ever found for any molecules in the PM, with a median diffusion coefficient (D) of 3.4 µm²/second, which was ∼10 times greater than that of non-raft phospholipid molecules (0.33 µm²/second), despite Bdp-Chol's probable association with raft domains. Furthermore, Bdp-Chol exhibited no sign of entrapment in time scales longer than 0.5 milliseconds. In the blebbed PM, where actin filaments were largely depleted, Bdp-Chol and Cy3-conjugated dioleoylphosphatidylethanolamine (Cy3-DOPE) diffused at comparable Ds (medians = 5.8 and 6.2 µm²/second, respectively), indicating that the actin-based membrane skeleton reduces the D of Bdp-Chol only by a factor of ∼2 from that in the blebbed PM, whereas it reduces the D of Cy3-DOPE by a factor of ∼20. These results are consistent with the previously proposed model, in which the PM is compartmentalized by the actin-based membrane-skeleton fence and its associated transmembrane picket proteins for the macroscopic diffusion of all of the membrane molecules, and suggest that the probability of Bdp-Chol passing through the compartment boundaries, once it enters the boundary, is ∼10× greater than that of Cy3-DOPE. Since the compartment sizes are greater than those of the putative raft domains, we conclude that raft domains coexist with membrane-skeleton-induced compartments and are contained within them.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.