Evidence map›Paper›PMID 24506189›Full record

ArticleACS chemical biology2014

Genetic incorporation of histidine derivatives using an engineered pyrrolysyl-tRNA synthetase.

Han Xiao, Francis B Peters, Peng-Yu Yang, Sean Reed, Johnathan R Chittuluru, Peter G Schultz

Abstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Han XiaoDepartment of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute , 10550 North Torrey Pines Road, La Jolla, California 92037, United States.
Francis B Peters
Peng-Yu Yang
Sean Reed
Johnathan R Chittuluru
Peter G Schultz

Funding

Ribosomal Synthesis of Peptides with Unnatural Building BlocksR01GM097206 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI SCHULTZ, PETER G · 2011 to 2014
$1.4M
NIGMS NIH HHS GM097206NIGMS NIH HHS R01 GM097206
6 · The paper itself

Abstract

A polyspecific amber suppressor aminoacyl-tRNA synthetase/tRNA pair was evolved that genetically encodes a series of histidine analogues in both Escherichia coli and mammalian cells. In combination with tRNACUA(Pyl), a pyrrolysyl-tRNA synthetase mutant was able to site-specifically incorporate 3-methyl-histidine, 3-pyridyl-alanine, 2-furyl-alanine, and 3-(2-thienyl)-alanine into proteins in response to an amber codon. Substitution of His66 in the blue fluorescent protein (BFP) with these histidine analogues created mutant proteins with distinct spectral properties. This work further expands the structural and chemical diversity of unnatural amino acids (UAAs) that can be genetically encoded in prokaryotic and eukaryotic organisms and affords new probes of protein structure and function.

Indexed as

AlanineAmino Acyl-tRNA SynthetasesEscherichia coliHistidineLuminescent ProteinsModels, MolecularMutant ProteinsMutationProtein Engineering3-pyridylalanineAlanineAmino Acyl-tRNA SynthetasesHistidineLuminescent ProteinsMutant Proteins

Identifiers

PMID24506189
PMCPMC4033645

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.