Evidence map›Paper›PMID 24505547›Full record

ArticleInternational journal of hematology-oncology and stem cell research2014

The Effect of miR-210 Up-regulation on Proliferation and Survival of Mouse Bone Marrow Derived Mesenchymal Stem Cell.

Neda Minayi, Shaban Alizadeh, Hosein Dargahi, Masoud Soleimani, Zahra Kashani Khatib, Behnoosh Tayebi, Mozhde Mohammadian, Sadegh Alijani, Farshid Karami

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PubMed Full text at PMC
In one paragraph

Article in International journal of hematology-oncology and stem cell research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Neda MinayiHematology Department, Allied Medical School, Tehran University of Medical sciences, Tehran, Iran.
Shaban AlizadehHematology Department, Allied Medical School, Tehran University of Medical sciences, Tehran, Iran.
Hosein DargahiHealth care services Department, Medical School, Tehran University of Medical sciences, Tehran, Iran.
Masoud SoleimaniHematology Department, Tarbiat Modares University, Tehran, Iran.
Zahra Kashani KhatibHematology Department, Tarbiat Modares University, Tehran, Iran.
Behnoosh TayebiHematology Department, Qaem Hospital, Mashad University of Medical sciences, Mashhad, Iran.
Mozhde MohammadianHematology Department, Tabriz University of Medical sciences, Tabriz, Iran.
Sadegh AlijaniHematology Department, Allied Medical School, Tehran University of Medical sciences, Tehran, Iran.
Farshid KaramiHematology Department, Allied Medical School, Tehran University of Medical sciences, Tehran, Iran.
Tarbiat Modares University · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBone marrow derived mesenchymal stem cells (MSCs) are a population of multipotent progenitors which have the capacity of proliferation and differentiation into mesenchymal lineage cells. Hypoxia could promote the proliferation of MSCs. Micro-RNAs are endogenous RNAs that can play an important role in some processes such as proliferation and differentiation. MiR-210 could help for better proliferation of MSCs since this miRNA could activate HIF pathway. In current study we investigated if MSCs can preserve their differentiation and proliferation ability under normoxic conditions by upregulation of miR-210. MATERIALS AND

methodsMSCs isolated from C57 BL/6 mice by flushing it's femurs into the cell culture media. After 72 hours, MSCs which are plastic adherent cells were attached to the flask and non-adherent cells were removed. Subsequently, MSCs induced to differentiate into osteocytes and adipocytes with specific differentiation media in order to confirm their identity and multipotency. Then miR-210 was inserted in Lentiviruse vectors and affected MSCs. In each passage, the number and viability of cells were evaluated.

resultsComparison between miR-210 infected MSCs with control cells showed that miR-210 has ability to increase proliferation of MSCs significantly.

conclusionWe showed that miR-210 has ability to induce proliferation of MSCs without any negative effect on their differentiation abilities. Further studies are needed for evaluation of probable effects of miR-210 mechanisms on MSCs proliferation.

Indexed as

HIF-1αMesenchymal stem cellsMiR-210

Identifiers

PMID24505547
PMCPMC3913157
OpenAlexW2118520307

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.