ArticlePloS one2014
Mechanisms of DNA damage response to targeted irradiation in organotypic 3D skin cultures.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Nano-Topography Enhanced Topological-Cell-Analysis in Radiation-Therapy.Advanced healthcare materials · 2025Review
- Post-Excision Soft X-Ray Radiotherapy for Keloids: Experience in a Tertiary Referral Center.Dermatology (Basel, Switzerland) · 2024Article
- 3D Cell Models in Radiobiology: Improving the Predictive Value of In Vitro Research.International journal of molecular sciences · 2023Review
- Skin Immuno-CometChip in 3D vs. 2D Cultures to Screen Topical Toxins and Skin-Specific Cytochrome Inducers.Genes · 2023Article
- 3D Breast Tumor Models for Radiobiology Applications.Cancers · 2021Review
- Rapid Evaluation of Novel Therapeutic Strategies Using a 3D Collagen-Based Tissue-Like Model.Frontiers in bioengineering and biotechnology · 2021Article
- Xenobiotic Receptors and Their Mates in Atopic Dermatitis.International journal of molecular sciences · 2019Review
- Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models.Scientific reports · 2017Article
- Repeated short climatic change affects the epidermal differentiation program and leads to matrix remodeling in a human organotypic skin model.Clinical, cosmetic and investigational dermatology · 2017Article
- Period2 downregulation inhibits glioma cell apoptosis by activating the MDM2-TP53 pathway.Oncotarget · 2016Article
- The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.Oxidative medicine and cellular longevity · 2016Article
- The combined effects of irradiation and herpes simplex virus type 1 infection on an immortal gingival cell line.Virology journal · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
DNA damage (caused by direct cellular exposure and bystander signaling) and the complex pathways involved in its repair are critical events underpinning cellular and tissue response following radiation exposures. There are limited data addressing the dynamics of DNA damage induction and repair in the skin particularly in areas not directly exposed. Here we investigate the mechanisms regulating DNA damage, repair, intracellular signalling and their impact on premature differentiation and development of inflammatory-like response in the irradiated and surrounding areas of a 3D organotypic skin model. Following localized low-LET irradiation (225 kVp X-rays), low levels of 53BP1 foci were observed in the 3D model (3.8±0.28 foci/Gy/cell) with foci persisting and increasing in size up to 48 h post irradiation. In contrast, in cell monolayers 14.2±0.6 foci/Gy/cell and biphasic repair kinetics with repair completed before 24 h was observed. These differences are linked to differences in cellular status with variable level of p21 driving apoptotic signalling in 2D and accelerated differentiation in both the directly irradiated and bystander areas of the 3D model. The signalling pathways utilized by irradiated keratinocytes to induce DNA damage in non-exposed areas of the skin involved the NF-κB transcription factor and its downstream target COX-2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.