ArticleNature protocols2014
Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 133 citations in OpenAlex.
- Tissue composition shapes differential skeletal integration strategies during axolotl limb regeneration.Nature communications · 2026Article
- Protocol for non-invasive genotyping of axolotls using skin swabbing.STAR protocols · 2026Article
- tBid-Mediated Genetic Ablation of Connective Tissue Cells Reveals Their Key Regulatory Function During Limb Regeneration in Axolotls.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- A mammalian-like piRNA pathway in Axolotl reveals the origins of piRNA-directed DNA methylation.The EMBO journal · 2026Article
- Axolotls retain fertility throughout lifespan.BMC biology · 2026Article
- Two axolotl-adapted cell-ablation platforms reveal macrophage-dependent processes essential for spinal-cord and skeletal regeneration.bioRxiv : the preprint server for biology · 2026Article
- Macrophage adaptation to hypoxia in the tuberculous granuloma potentiates mycobacterium-induced mitochondrial damage and granuloma necrosis.bioRxiv : the preprint server for biology · 2026Article
- Divergent stem cell mechanisms govern the primary body axis and appendage regeneration in the axolotl.Science advances · 2026Article
- Ependymoglial cells are critical for cortex regeneration in axolotls.Nature communications · 2026Article
- Article
- Management of Hydrocoelom in an Axolotl (Ambystoma mexicanum).Veterinary medicine and science · 2025Article
- Adeno-associated viral tools to trace neural development and connectivity across amphibians.Developmental cell · 2025Article
- Systemic and local lipid adaptations underlie regeneration in Drosophila melanogaster and Ambystoma mexicanum.NPJ Regenerative medicine · 2024Article
- Axolotl mandible regeneration occurs through mechanical gap closure and a shared regenerative program with the limb.Disease models & mechanisms · 2024Article
- The salamander limb: a perfect model to understand imperfect integration during skeletal regeneration.Biology open · 2024Review
- Collagen IV of basement membranes: II. Emergence of collagen IVThe Journal of biological chemistry · 2023Article
- Conserved enhancers control notochord expression of vertebrate Brachyury.Nature communications · 2023Article
- Multi-species atlas resolves an axolotl limb development and regeneration paradox.Nature communications · 2023Article
- Differential effects of 3,5-T2 and T3 on the gill regeneration and metamorphosis of theFrontiers in endocrinology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The axolotl (Mexican salamander, Ambystoma mexicanum) has become a very useful model organism for studying limb and spinal cord regeneration because of its high regenerative capacity. Here we present a protocol for successfully mating and breeding axolotls in the laboratory throughout the year, for metamorphosing axolotls by a single i.p. injection and for axolotl transgenesis using I-SceI meganuclease and the mini Tol2 transposon system. Tol2-mediated transgenesis provides different features and advantages compared with I-SceI-mediated transgenesis, and it can result in more than 30% of animals expressing the transgene throughout their bodies so that they can be directly used for experimentation. By using Tol2-mediated transgenesis, experiments can be performed within weeks (e.g., 5-6 weeks for obtaining 2-3-cm-long larvae) without the need to establish germline transgenic lines (which take 12-18 months). In addition, we describe here tamoxifen-induced Cre-mediated recombination in transgenic axolotls.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.