ArticlePloS one2014
Isolation, purification and properties of an R-Phycocyanin from the phycobilisomes of a marine red macroalga Polysiphonia urceolata.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 48 citations in OpenAlex.
- Antioxidant Efficacy of aMarine drugs · 2022Trial
- Progress in the Synthesis and Application of Phycocyanin-Based Nanoparticles.Topics in current chemistry (Cham) · 2025Review
- Probing the structural stability of R-phycocyanin under pressure.Protein science : a publication of the Protein Society · 2024Article
- Spectral Characteristics, In Silico Perspectives, Density Functional Theory (DFT), and Therapeutic Potential of Green-Extracted Phycocyanin fromInternational journal of molecular sciences · 2024Article
- Studying the impact of phycoerythrin on antioxidant and antimicrobial activity of the fresh rainbow trout fillets.Scientific reports · 2024Article
- Recent Progress of Natural and Recombinant Phycobiliproteins as Fluorescent Probes.Marine drugs · 2023Review
- Microalgae: A Promising Source of Bioactive Phycobiliproteins.Marine drugs · 2023Review
- Cyano-Phycocyanin: Mechanisms of Action on Human Skin and Future Perspectives in Medicine.Plants (Basel, Switzerland) · 2022Review
- Phycocyanin Extracted from Oscillatoria minima Shows Antimicrobial, Algicidal, and Antiradical Activities: In silico and In vitro Analysis.Anti-inflammatory & anti-allergy agents in medicinal chemistry · 2020Article
- Identification and antioxidant activity of synthetic peptides from phycobiliproteins of Pyropia yezoensis.International journal of molecular medicine · 2018Article
- Phycocyanin: A Potential Drug for Cancer Treatment.Journal of Cancer · 2017Review
- Characterization, Preparation, and Purification of Marine Bioactive Peptides.BioMed research international · 2017Review
- Isolation, purification and properties of an R-phycocyanin from the phycobilisomes of a marine red macroalga Polysiphonia urceolata.PloS one · 2014Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phycobilisomes were prepared from a marine red macroalga Polysiphonia urceolata (P. urceolata) by sucrose step-gradient ultracentrifugation. From the prepared phycobilisomes, an R-phycocyanin was isolated by gel filtration on Sephadex G-150 and then purified by ion exchange chromatography on DEAE-Sepharose Fast Flow and native polyacrylamide gel electrophoresis (PAGE) performed in neutral buffer systems. The purified R-phycocyanins showed not only a homogeneous trimer of 136 kDa in gel filtration and a single band in native PAGE, but also exhibited one band at about pH 5.7 in native isoelectric focusing (IEF). By a gradient SDS-PAGE the purified R-phycocyanin was determined to contain one α subunit of 17.5 kDa (α (17.5)) and two β subunits of 21.3 kDa and 22.6 kDa (β (21.3) and β (22.6)). The analysis from denaturing isoelectric focusing and two-dimension PAGE demonstrated that α (17.5), β (21.3) and β (22.6) had their pIs of 6.4, 5.3 and 5.4, respectively. Furthermore, mass spectroscopy analysis of β (21.3) and β (22.6) by MALDI-TOF mass spectrometry demonstrated the two β subunits had differences in peptide mass fingerprinting. These results revealed that the prepared R-phycocyanins were composed of one α and two β subunits. and , which have a structural foundation to show their pIs too close for them to be definitely resolved by native IEF, are postulated to be the most possible trimeric forms of the R-phycocyanins prepared from the phycobilisomes of P. urceolata.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.