ArticleScientific reports2014
Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 114 citations in OpenAlex.
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- Kinetic Analysis of SARS-CoV-2 S1-Integrin Binding Using Live-Cell, Label-Free Optical Biosensing.Biosensors · 2025Article
- Label-Free Single-Cell Cancer Classification from the Spatial Distribution of Adhesion Contact Kinetics.ACS sensors · 2024Article
- Optical sensor reveals the hidden influence of cell dissociation on adhesion measurements.Scientific reports · 2024Article
- Single-cell classification based on label-free high-resolution optical data of cell adhesion kinetics.Scientific reports · 2024Article
- Label-free biomolecular and cellular methods in small molecule epigallocatechin-gallate research.Heliyon · 2024Review
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- Polarized focal adhesion kinase activity within a focal adhesion during cell migration.Nature chemical biology · 2023Article
- Microfluidic Systems for Neural Cell Studies.Bioengineering (Basel, Switzerland) · 2023Review
- The Use of Sensors in Blood-Brain Barrier-on-a-Chip Devices: Current Practice and Future Directions.Biosensors · 2023Review
- Application of biosensors in cancers, an overview.Frontiers in bioengineering and biotechnology · 2023Review
- Intraprocedural endothelial cell seeding of arterial stents via biotin/avidin targeting mitigates in-stent restenosis.Scientific reports · 2022Article
- Single-cell adhesivity distribution of glycocalyx digested cancer cells from high spatial resolution label-free biosensor measurements.Matrix biology plus · 2022Article
- Design and Synthesis of Porphyrin-Nitrilotriacetic Acid Dyads with Potential Applications in Peptide Labeling through Metallochelate Coupling.ACS omega · 2022Article
- Natural Compounds as Target Biomolecules in Cellular Adhesion and Migration: From Biomolecular Stimulation to Label-Free Discovery and Bioactivity-Based Isolation.Biomedicines · 2021Review
- Single-cell adhesion strength and contact density drops in the M phase of cancer cells.Scientific reports · 2021Article
- Red Blood Cells: Tethering, Vesiculation, and Disease in Micro-Vascular Flow.Diagnostics (Basel, Switzerland) · 2021Article
- Leaky and waveguide modes in biperiodic holograms.Scientific reports · 2021Article
- Review
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel high-throughput label-free resonant waveguide grating (RWG) imager biosensor, the Epic® BenchTop (BT), was utilized to determine the dependence of cell spreading kinetics on the average surface density (v(RGD)) of integrin ligand RGD-motifs. v(RGD) was tuned over four orders of magnitude by co-adsorbing the biologically inactive PLL-g-PEG and the RGD-functionalized PLL-g-PEG-RGD synthetic copolymers from their mixed solutions onto the sensor surface. Using highly adherent human cervical tumor (HeLa) cells as a model system, cell adhesion kinetic data of unprecedented quality were obtained. Spreading kinetics were fitted with the logistic equation to obtain the spreading rate constant (r) and the maximum biosensor response (Δλmax), which is assumed to be directly proportional to the maximum spread contact area (Amax). r was found to be independent of the surface density of integrin ligands. In contrast, Δλmax increased with increasing RGD surface density until saturation at high densities. Interpreting the latter behavior with a simple kinetic mass action model, a 2D dissociation constant of 1753 ± 243 μm(-2) (corresponding to a 3D dissociation constant of ~30 μM) was obtained for the binding between RGD-specific integrins embedded in the cell membrane and PLL-g-PEG-RGD. All of these results were obtained completely noninvasively without using any labels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.