ArticleNucleic acids research2014
The FurA regulon in Anabaena sp. PCC 7120: in silico prediction and experimental validation of novel target genes.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- From photosynthetic electron flow to gene regulation: redox signal transduction in cyanobacteria.FEMS microbiology reviews · 2026Review
- Hacking heterocysts: advances in the genetic regulation of heterocyst differentiation.Archives of microbiology · 2025Review
- NsrM (All0345) and NsrX (Alr1976), two FurC (PerR)-targeted transcriptional regulators, modulate nitrogen metabolism and heterocyst differentiation genes in the cyanobacteriumMicrobiology spectrum · 2025Article
- Towards the control of biofilm formation in Anabaena (Nostoc) sp. PCC7120: novel insights into the genes involved and their regulation.The New phytologist · 2025Article
- Article
- The Role of FtsH Complexes in the Response to Abiotic Stress in Cyanobacteria.Plant & cell physiology · 2024Review
- Genome-wide identification and characterization of Fur-binding sites in the cyanobacteria Synechocystis sp. PCC 6803 and PCC 6714.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021Article
- Cyanochelins, an Overlooked Class of Widely Distributed Cyanobacterial Siderophores, Discovered by Silent Gene Cluster Awakening.Applied and environmental microbiology · 2021Article
- Article
- Free Rather Than Total Iron Content Is Critically Linked to the Fur Physiology inFrontiers in microbiology · 2020Article
- Depletion of the FtsH1/3 Proteolytic Complex Suppresses the Nutrient Stress Response in the CyanobacteriumThe Plant cell · 2019Article
- Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects ofApplied and environmental microbiology · 2018Article
- Multiplicity and specificity of siderophore uptake in the cyanobacterium Anabaena sp. PCC 7120.Plant molecular biology · 2016Article
- Cysteine Mutational Studies Provide Insight into a Thiol-Based Redox Switch Mechanism of Metal and DNA Binding in FurA from Anabaena sp. PCC 7120.Antioxidants & redox signaling · 2016Article
- Article
- Interdependence of tetrapyrrole metabolism, the generation of oxidative stress and the mitigative oxidative stress response.Redox biology · 2015Review
- Fur-type transcriptional repressors and metal homeostasis in the cyanobacterium Synechococcus sp. PCC 7002.Frontiers in microbiology · 2015Article
- The long goodbye: the rise and fall of flavodoxin during plant evolution.Journal of experimental botany · 2014Review
- Regulatory Tools for Controlling Gene Expression in Cyanobacteria.Advances in experimental medicine and biologyReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the filamentous cyanobacterium Anabaena sp. PCC 7120, the ferric uptake regulator FurA functions as a global transcriptional regulator. Despite several analyses have focused on elucidating the FurA-regulatory network, the number of target genes described for this essential transcription factor is limited to a handful of examples. In this article, we combine an in silico genome-wide predictive approach with experimental determinations to better define the FurA regulon. Predicted FurA-binding sites were identified upstream of 215 genes belonging to diverse functional categories including iron homeostasis, photosynthesis and respiration, heterocyst differentiation, oxidative stress defence and light-dependent signal transduction mechanisms, among others. The probabilistic model proved to be effective at discerning FurA boxes from non-cognate sequences, while subsequent electrophoretic mobility shift assay experiments confirmed the in vitro specific binding of FurA to at least 20 selected predicted targets. Gene-expression analyses further supported the dual role of FurA as transcriptional modulator that can act both as repressor and as activator. In either role, the in vitro affinity of the protein to its target sequences is strongly dependent on metal co-regulator and reducing conditions, suggesting that FurA couples in vivo iron homeostasis and the response to oxidative stress to major physiological processes in cyanobacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.