ArticleBioTechniques2014
Comparative analysis of mitosis-specific antibodies for bulk purification of mitotic populations by fluorescence-activated cell sorting.
Article in BioTechniques, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- DiMeLo-cito: a one-tube protocol for mapping protein-DNA interactions reveals CTCF bookmarking in mitosis.bioRxiv : the preprint server for biology · 2025Article
- Genome folding principles uncovered in condensin-depleted mitotic chromosomes.Nature genetics · 2024Article
- Genome folding principles revealed in condensin-depleted mitotic chromosomes.bioRxiv : the preprint server for biology · 2023Article
- GATA2 mitotic bookmarking is required for definitive haematopoiesis.Nature communications · 2023Article
- CTCF and transcription influence chromatin structure re-configuration after mitosis.Nature communications · 2021Article
- Interrogating Histone Acetylation and BRD4 as Mitotic Bookmarks of Transcription.Cell reports · 2019Article
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- Condensin recruitment to chromatin is inhibited by Chk2 kinase in response to DNA damage.Cell cycle (Georgetown, Tex.) · 2016Article
- A hyperactive transcriptional state marks genome reactivation at the mitosis-G1 transition.Genes & development · 2016Article
- Genome accessibility is widely preserved and locally modulated during mitosis.Genome research · 2015Article
- Mitotic catenation is monitored and resolved by a PKCε-regulated pathway.Nature communications · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Mitosis entails complex chromatin changes that have garnered increasing interest from biologists who study genome structure and regulation-fields that are being advanced by high-throughput sequencing (Seq) technologies. The application of these technologies to study the mitotic genome requires large numbers of highly pure mitotic cells, with minimal contamination from interphase cells, to ensure accurate measurement of phenomena specific to mitosis. Here, we optimized a fluorescence-activated cell sorting (FACS)-based method for isolating formaldehyde-fixed mitotic cells--at virtually 100% mitotic purity and in quantities sufficient for high-throughput genomic studies. We compared several commercially available antibodies that react with mitosis-specific epitopes over a range of concentrations and cell numbers, finding antibody MPM2 to be the most robust and cost-effective.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.