ArticleRadiation research2014
Fast image analysis for the micronucleus assay in a fully automated high-throughput biodosimetry system.
Article in Radiation research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- RABiT-III: an Automated Micronucleus Assay at a Non-Specialized Biodosimetry Facility.Radiation research · 2024Article
- Rapid and automatic detection of micronuclei in binucleated lymphocytes image.Scientific reports · 2022Article
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- Fabric Phase Sorptive Extraction-A Metabolomic Preprocessing Approach for Ionizing Radiation Exposure Assessment.Journal of proteome research · 2019Article
- Article
- Automated Triage Radiation Biodosimetry: Integrating Imaging Flow Cytometry with High-Throughput Robotics to Perform the Cytokinesis-Block Micronucleus Assay.Radiation research · 2019Article
- Estimation of the effects of medical diagnostic radiation exposure based on DNA damage.Journal of radiation research · 2018Review
- RABiT-II: Implementation of a High-Throughput Micronucleus Biodosimetry Assay on Commercial Biotech Robotic Systems.Radiation research · 2017Article
- THE DECADE OF THE RABiT (2005-15).Radiation protection dosimetry · 2016Article
- In vitro RABiT measurement of dose rate effects on radiation induction of micronuclei in human peripheral blood lymphocytes.Radiation and environmental biophysics · 2016Article
- The cytokinesis-blocked micronucleus assay: dose-response calibration curve, background frequency in the population and dose estimation.Radiation and environmental biophysics · 2016Article
- An automated imaging system for radiation biodosimetry.Microscopy research and technique · 2015Article
- The RABiT: high-throughput technology for assessing global DSB repair.Radiation and environmental biophysics · 2014Article
- Automated analysis of the cytokinesis-block micronucleus assay for radiation biodosimetry using imaging flow cytometry.Radiation and environmental biophysics · 2014Article
- The RABiT: a rapid automated biodosimetry tool for radiological triage. II. Technological developments.International journal of radiation biology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The development of, and results from an image analysis system are presented for automated detection and scoring of micronuclei in human peripheral blood lymphocytes. The system is part of the Rapid Automated Biodosimetry Tool, which was developed at the Center for High-Throughput Minimally Invasive Radiation Biodosimetry for rapid radiation dose assessment of many individuals based on single fingerstick samples of blood. Blood lymphocytes were subjected to the cytokinesis-block micronucleus assay and the images of cell cytoplasm and nuclei are analyzed to estimate the frequency of micronuclei in binucleated cells. We describe an algorithm that is based on dual fluorescent labeling of lymphocytes with separate analysis of images of cytoplasm and nuclei. To evaluate the performance of the system, blood samples of seven healthy donors were irradiated in vitro with doses from 0-10 Gy and dose-response curves of micronuclei frequencies were generated. To establish the applicability of the system to the detection of high doses, the ratios of mononucleated cells to binucleated cells were determined for three of the donors. All of the dose-response curves generated automatically showed clear dose dependence and good correlation (R(2) from 0.914-0.998) with the results of manual scoring.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.