ArticleInterface focus2014
Neural cell alignment by patterning gradients of the extracellular matrix protein laminin.
Article in Interface focus, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Cell alignment on novel polymeric micro-hollow fiber membranes for neural and musculoskeletal tissue engineering.Cell and tissue research · 2026Article
- Continuous protein-density gradients: A new approach to correlate physical cues with cell response.PNAS nexus · 2024Article
- The first embryo, the origin of cancer and animal phylogeny. II. The neoplastic process as an evolutionary engine.Journal of biosciences · 2024Article
- Synergy of Nanotopography and Electrical Conductivity of PEDOT/PSS for Enhanced Neuronal Development.ACS applied materials & interfaces · 2023Article
- PEDOT: PSS promotes neurogenic commitment of neural crest-derived stem cells.Frontiers in physiology · 2022Article
- Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System.Pharmaceutics · 2021Review
- Amphiphilic cationic cyclodextrin nanovesicles: a versatile cue for guiding cell adhesion.Nanoscale advances · 2020Article
- Enzyme-Mediated Conjugation of Peptides to Silk Fibroin for Facile Hydrogel Functionalization.Annals of biomedical engineering · 2020Article
- Controlled Arrangement of Neuronal Cells on Surfaces Functionalized with Micropatterned Polymer Brushes.ACS omega · 2018Article
- Surface Modification of Polymer Substrates for Biomedical Applications.Materials (Basel, Switzerland) · 2017Review
- Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells.Journal of visualized experiments : JoVE · 2016Article
- Functionalized α-Helical Peptide Hydrogels for Neural Tissue Engineering.ACS biomaterials science & engineering · 2015Article
- Coextruded, aligned, and gradient-modified poly(ε-caprolactone) fibers as platforms for neural growth.Biomacromolecules · 2015Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anisotropic orientation and accurate positioning of neural cells is achieved by patterning stripes of the extracellular matrix protein laminin on the surface of polystyrene tissue culture dishes by micromoulding in capillaries (MIMICs). Laminin concentration decreases from the entrance of the channels in contact with the reservoir towards the end. Immunofluorescence analysis of laminin shows a decreasing gradient of concentration along the longitudinal direction of the stripes. The explanation is the superposition of diffusion and convection of the solute, the former dominating at length scales near the entrance (characteristic length around 50 μm), the latter further away (length scale in excess of 900 μm). These length scales are independent of the channel width explored from about 15 to 45 μm. Neural cells are randomly seeded and selectively adhere to the pattern, leaving the unpatterned areas depleted even upon 6 days of incubation. Cell alignment was assessed by the orientation of the long axis of the 4',6-diamidino-2-phenylindole-stained nuclei. Samples on patterned the laminin area exhibit a large orientational order parameter. As control, cells on the unpatterned laminin film exhibit no preferential orientation. This implies that the anisotropy of laminin stripes is an effective chemical stimulus for cell recruiting and alignment.
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Registered trials
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