ArticleMolecular biology of the cell2014
New single-molecule speckle microscopy reveals modification of the retrograde actin flow by focal adhesions at nanometer scales.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed.
- The mechanism of dynamic steady states in lamellipodia.PLoS computational biology · 2025Article
- Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics.iScience · 2025Article
- Force transmission by retrograde actin flow-induced dynamic molecular stretching of Talin.Nature communications · 2023Article
- Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay.Biophysical journal · 2023Article
- Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay.bioRxiv : the preprint server for biology · 2023Article
- Engineered fast-dissociating antibody fragments for multiplexed super-resolution microscopy.Cell reports methods · 2022Article
- Extraction of accurate cytoskeletal actin velocity distributions from noisy measurements.Nature communications · 2022Article
- Article
- Discrete mechanical model of lamellipodial actin network implements molecular clutch mechanism and generates arcs and microspikes.PLoS computational biology · 2021Article
- Filament-guided filament assembly provides structural memory of filament alignment during cytokinesis.Developmental cell · 2021Article
- WAVE1 and WAVE2 have distinct and overlapping roles in controlling actin assembly at the leading edge.Molecular biology of the cell · 2020Article
- Phospholipid phosphatase related 1 (PLPPR1) increases cell adhesion through modulation of Rac1 activity.Experimental cell research · 2020Article
- Quantitative high-precision imaging of myosin-dependent filamentous actin dynamics.Journal of muscle research and cell motility · 2020Review
- Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis.eLife · 2019Article
- Lamellipodium tip actin barbed ends serve as a force sensor.Genes to cells : devoted to molecular & cellular mechanisms · 2019Article
- Convection-Induced Biased Distribution of Actin Probes in Live Cells.Biophysical journal · 2019Article
- New neurons use Slit-Robo signaling to migrate through the glial meshwork and approach a lesion for functional regeneration.Science advances · 2018Article
- Myosin-dependent actin stabilization as revealed by single-molecule imaging of actin turnover.Molecular biology of the cell · 2018Article
- Helical rotation of the diaphanous-related formin mDia1 generates actin filaments resistant to cofilin.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Reconsidering an active role for G-actin in cytoskeletal regulation.Journal of cell science · 2018Review
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7 authors.
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Abstract
Speckle microscopy directly visualizes the retrograde actin flow, which is believed to promote cell-edge protrusion when linked to focal adhesions (FAs). However, it has been argued that, due to rapid actin turnover, the use of green fluorescent protein-actin, the lack of appropriate analysis algorithms, and technical difficulties, speckle microscopy does not necessarily report the flow velocities of entire actin populations. In this study, we developed a new, user-friendly single-molecule speckle (SiMS) microscopy using DyLight dye-labeled actin. Our new SiMS method enables in vivo nanometer-scale displacement analysis with a low localization error of ±8-8.5 nm, allowing accurate flow-velocity measurement for actin speckles with lifetime <5 s. In lamellipodia, both short- and long-lived F-actin molecules flow with the same speed, indicating they are part of a single actin network. These results do not support coexistence of F-actin populations with different flow speeds, which is referred to as the lamella hypothesis. Mature FAs, but not nascent adhesions, locally obstruct the retrograde flow. Interestingly, the actin flow in front of mature FAs is fast and biased toward FAs, suggesting that mature FAs attract the flow in front and actively remodel the local actin network.
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