ArticleMolecular biology of the cell2014
Katanin maintains meiotic metaphase chromosome alignment and spindle structure in vivo and has multiple effects on microtubules in vitro.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 57 citations in OpenAlex.
- Differential contributions of β-tubulin isotypes to acentrosomal oocyte meiosis in C. elegans.The Journal of cell biology · 2026Article
- Microtubule lattice defects facilitate spastin-mediated severing.Journal of cell science · 2026Article
- Differential contributions of β-tubulin isotypes to acentrosomal oocyte meiosis inbioRxiv : the preprint server for biology · 2025Article
- Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase ofLife science alliance · 2024Article
- Microtubule length correlates with spindle length in C. elegans meiosis.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase ofbioRxiv : the preprint server for biology · 2024Article
- Microtubule-binding domains in Katanin p80 subunit are essential for severing activity in C. elegans.The Journal of cell biology · 2024Article
- C. elegans XMAP215/ZYG-9 and TACC/TAC-1 act at multiple times during oocyte meiotic spindle assembly and promote both spindle pole coalescence and stability.PLoS genetics · 2023Article
- Combinatorial and antagonistic effects of tubulin glutamylation and glycylation on katanin microtubule severing.Developmental cell · 2022Article
- ZYG-9ch-TOG promotes the stability of acentrosomal poles via regulation of spindle microtubules in C. elegans oocyte meiosis.PLoS genetics · 2022Article
- Cohesin is required for meiotic spindle assembly independent of its role in cohesion in C. elegans.PLoS genetics · 2022Article
- STRIPAK regulation of katanin microtubule severing in the Caenorhabditis elegans embryo.Genetics · 2022Article
- Article
- An anchoring complex recruits katanin for microtubule severing at the plant cortical nucleation sites.Nature communications · 2021Article
- Article
- Impact of the 'tubulin economy' on the formation and function of the microtubule cytoskeleton.Current opinion in cell biology · 2021Review
- Cutting, Amplifying, and Aligning Microtubules with Severing Enzymes.Trends in cell biology · 2021Review
- Evidence for anaphase pulling forces during C. elegans meiosis.The Journal of cell biology · 2020Article
- C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion.PLoS genetics · 2020Article
- Katanin Grips the β-Tubulin Tail through an Electropositive Double Spiral to Sever Microtubules.Developmental cell · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Assembly of Caenorhabditis elegans female meiotic spindles requires both MEI-1 and MEI-2 subunits of the microtubule-severing ATPase katanin. Strong loss-of-function mutants assemble apolar intersecting microtubule arrays, whereas weaker mutants assemble bipolar meiotic spindles that are longer than wild type. To determine whether katanin is also required for spindle maintenance, we monitored metaphase I spindles after a fast-acting mei-1(ts) mutant was shifted to a nonpermissive temperature. Within 4 min of temperature shift, bivalents moved off the metaphase plate, and microtubule bundles within the spindle lengthened and developed a high degree of curvature. Spindles eventually lost bipolar structure. Immunofluorescence of embryos fixed at increasing temperature indicated that MEI-1 was lost from spindle microtubules before loss of ASPM-1, indicating that MEI-1 and ASPM-1 act independently at spindle poles. We quantified the microtubule-severing activity of purified MEI-1/MEI-2 complexes corresponding to six different point mutations and found a linear relationship between microtubule disassembly rate and meiotic spindle length. Previous work showed that katanin is required for severing at points where two microtubules intersect in vivo. We show that purified MEI-1/MEI-2 complexes preferentially sever at intersections between two microtubules and directly bundle microtubules in vitro. These activities could promote parallel/antiparallel microtubule organization in meiotic spindles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.