ArticleBlood2014
Quantitative phosphoproteomics unveils temporal dynamics of thrombin signaling in human endothelial cells.
Article in Blood, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Phosphoproteomics in Vascular Biology and Disease: Illuminating Signaling in the Vessel.Arteriosclerosis, thrombosis, and vascular biology · 2026Review
- An Integrative Omics and Network Pharmacology Approach to Evaluate the Antiulcer Activity ofACS omega · 2025Article
- Interrogating endothelial barrier regulation by temporally resolved kinase network generation.Life science alliance · 2024Article
- Serine phosphorylation of the RhoGEF Trio stabilizes endothelial cell-cell junctions.Small GTPases · 2023Article
- Phosphoproteomics data-driven signalling network inference: Does it work?Computational and structural biotechnology journal · 2023Article
- Exploiting polypharmacology to dissect host kinases and kinase inhibitors that modulate endothelial barrier integrity.Cell chemical biology · 2021Article
- Heat shock protein 27 activity is linked to endothelial barrier recovery after proinflammatory GPCR-induced disruption.Science signaling · 2021Article
- A thrombin-PAR1/2 feedback loop amplifies thromboinflammatory endothelial responses to the viral RNA analogue poly(I:C).Blood advances · 2021Article
- The Role of Thrombin in Brain Injury After Hemorrhagic and Ischemic Stroke.Translational stroke research · 2021Review
- The Extraordinary Role of Extracellular RNA in Arteriogenesis, the Growth of Collateral Arteries.International journal of molecular sciences · 2019Review
- C-type lectin domain group 14 proteins in vascular biology, cancer and inflammation.The FEBS journal · 2019Review
- qPhos: a database of protein phosphorylation dynamics in humans.Nucleic acids research · 2019Article
- Macrophage migration inhibitory factor is critical for dengue NS1-induced endothelial glycocalyx degradation and hyperpermeability.PLoS pathogens · 2018Article
- Plasma microRNAs levels are different between pulmonary and extrapulmonary ARDS patients: a clinical observational study.Annals of intensive care · 2018Article
- Protease-Activated Receptor-1 Signaling: The Big Picture.Arteriosclerosis, thrombosis, and vascular biology · 2017Article
- Modulating Protein-Protein Interactions of the Mitotic Polo-like Kinases to Target Mutant KRAS.Cell chemical biology · 2017Article
- Using omics approaches to understand pulmonary diseases.Respiratory research · 2017Review
- Targeting PAR1: Now What?Trends in pharmacological sciences · 2017Review
- Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer.Cell reports · 2017Article
- The Initiator Methionine tRNA Drives Secretion of Type II Collagen from Stromal Fibroblasts to Promote Tumor Growth and Angiogenesis.Current biology : CB · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Thrombin is the key serine protease of the coagulation cascade and a potent trigger of protease-activated receptor 1 (PAR1)-mediated platelet aggregation. In recent years, PAR1 has become an appealing target for anticoagulant therapies. However, the inhibitors that have been developed so far increase bleeding risk in patients, likely because they interfere with endogenous PAR1 signaling in the endothelium. Because of its complexity, thrombin-induced signaling in endothelial cells has remained incompletely understood. Here, we have combined stable isotope amino acids in cell culture, affinity-based phosphopeptide enrichment, and high-resolution mass spectrometry and performed a time-resolved analysis of the thrombin-induced signaling in human primary endothelial cells. We identified 2224 thrombin-regulated phosphorylation sites, the majority of which have not been previously related to thrombin. Those sites were localized on proteins that are novel to thrombin signaling, but also on well-known players such as PAR1, Rho-associated kinase 2, phospholipase C, and proteins related to actin cytoskeleton, cell-cell junctions, and Weibel-Palade body release. Our study provides a unique resource of phosphoproteins and phosphorylation sites that may generate novel insights into an intimate understanding of thrombin-mediated PAR signaling and the development of improved PAR1 antagonists that affect platelet but not endothelial cell function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.