ArticleProceedings of the National Academy of Sciences of the United States of America2014
GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in organelles.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 67 citations in OpenAlex.
- Article
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- The Multifaceted Role of Calcium Signaling in Regulated Necrosis.Biomolecules · 2025Review
- Robust p53 phenotypes and prospective downstream targets in telomerase-immortalized human cells.Oncotarget · 2025Article
- Monitoring ER CaMethods in molecular biology (Clifton, N.J.) · 2025Article
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- Article
- The evolution of organellar calcium mapping technologies.Cell calcium · 2022Review
- Shaped by leaky ER: Homeostatic CaFrontiers in physiology · 2022Review
- Bioluminescent Sensors for CaFrontiers in bioengineering and biotechnology · 2021Article
- Direct monitoring of ER CaPflugers Archiv : European journal of physiology · 2020Article
- Live Mitochondrial or Cytosolic Calcium Imaging Using Genetically-encoded Cameleon Indicator in Mammalian Cells.Bio-protocol · 2020Article
- Imaging of Endoplasmic Reticulum CaFrontiers in endocrinology · 2020Article
- Mitochondrial CaFEBS letters · 2019Review
- Fluorescent probes for organelle-targeted bioactive species imaging.Chemical science · 2019Review
- Antimicrobial activity ofIndustrial crops and products · 2019Review
- Genetically Encoded Calcium Indicators: A New Tool in Renal Hypertension Research.Frontiers in medicine · 2019Review
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- The machineries, regulation and cellular functions of mitochondrial calcium.Nature reviews. Molecular cell biology · 2018Review
- Dual Color Sensors for Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells.Frontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically encoded calcium indicators allow monitoring subcellular Ca(2+) signals inside organelles. Most genetically encoded calcium indicators are fusions of endogenous calcium-binding proteins whose functionality in vivo may be perturbed by competition with cellular partners. We describe here a novel family of fluorescent Ca(2+) sensors based on the fusion of two Aequorea victoria proteins, GFP and apo-aequorin (GAP). GAP exhibited a unique combination of features: dual-excitation ratiometric imaging, high dynamic range, good signal-to-noise ratio, insensitivity to pH and Mg(2+), tunable Ca(2+) affinity, uncomplicated calibration, and targetability to five distinct organelles. Moreover, transgenic mice for endoplasmic reticulum-targeted GAP exhibited a robust long-term expression that correlated well with its reproducible performance in various neural tissues. This biosensor fills a gap in the actual repertoire of Ca(2+) indicators for organelles and becomes a valuable tool for in vivo Ca(2+) imaging applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.