ArticleThe ISME journal2014
Life in an unusual intracellular niche: a bacterial symbiont infecting the nucleus of amoebae.
Article in The ISME journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 58 citations in OpenAlex.
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- Single-cell sequencing reveals unexpected genetic diversity amongMicrobial genomics · 2026Article
- Experimental assessment of interactions between marine bacteria and model protists: from predator-prey relationships to bacterial-mediated lysis.Applied and environmental microbiology · 2025Article
- Paramoeba atlantica as a Natural Intracellular Niche for Vibrios in Marine Ecosystems.Environmental microbiology · 2025Article
- Protein import into bacterial endosymbionts and evolving organelles.The FEBS journal · 2025Review
- An Evolutionary-Focused Review of the Holosporales (Alphaproteobacteria): Diversity, Host Interactions, and Taxonomic Re-ranking as Holosporineae Subord. Nov.Microbial ecology · 2025Review
- Hepatincolaceae (Alphaproteobacteria) are Distinct From Holosporales and Independently Evolved to Associate With Ecdysozoa.Environmental microbiology · 2025Article
- An intranuclear bacterial parasite of deep-sea mussels expresses apoptosis inhibitors acquired from its host.Nature microbiology · 2024Article
- Review
- "Candidatus Intestinibacterium parameciiphilum"-member of the "Candidatus Paracaedibacteraceae" family (Alphaproteobacteria, Holosporales) inhabiting the ciliated protist Paramecium.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- The consequences of viral infection on protists.Communications biology · 2024Review
- The terrestrial isopod symbiont 'Candidatus Hepatincola porcellionum' is a potential nutrient scavenger related to Holosporales symbionts of protists.ISME communications · 2023Article
- Proposal of names for 329 higher rank taxa defined in the Genome Taxonomy Database under two prokaryotic codes.FEMS microbiology letters · 2023Article
- Gene Transfer Agents in Bacterial Endosymbionts of Microbial Eukaryotes.Genome biology and evolution · 2022Article
- Article
- Commensals Serve as Natural Barriers to Mammalian Cells during Acanthamoeba castellanii Invasion.Microbiology spectrum · 2021Article
- The Ecology and Evolution of Amoeba-Bacterium Interactions.Applied and environmental microbiology · 2021Review
- CARD-FISH in the Sequencing Era: Opening a New Universe of Protistan Ecology.Frontiers in microbiology · 2021Review
- Marine Bacteria Display Different Escape Mechanisms When Facing Their Protozoan Predators.Microorganisms · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Amoebae serve as hosts for various intracellular bacteria, including human pathogens. These microbes are able to overcome amoebal defense mechanisms and successfully establish a niche for replication, which is usually the cytoplasm. Here, we report on the discovery of a bacterial symbiont that is located inside the nucleus of its Hartmannella sp. host. This symbiont, tentatively named 'Candidatus Nucleicultrix amoebiphila', is only moderately related to known bacteria (∼90% 16S and 23S rRNA sequence similarity) and member of a novel clade of protist symbionts affiliated with the Rickettsiales and Rhodospirillales. Screening of 16S rRNA amplicon data sets revealed a broad distribution of these bacteria in freshwater and soil habitats. 'Candidatus Nucleicultrix amoebiphila' traffics within 6 h post infection to the host nucleus. Maximum infection levels are reached after 96-120 h, at which time point the nucleus is pronouncedly enlarged and filled with bacteria. Transmission of the symbionts occurs vertically upon host cell division but may also occur horizontally through host cell lysis. Although we observed no impact on the fitness of the original Hartmannella sp. host, the bacteria are rather lytic for Acanthamoeba castellanii. Intranuclear symbiosis is an exceptional phenomenon, and amoebae represent an ideal model system to further investigate evolution and underlying molecular mechanisms of these unique microbial associations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.