ArticleJournal of microbiology (Seoul, Korea)2014
Identification and characterization of ectoine biosynthesis genes and heterologous expression of the ectABC gene cluster from Halomonas sp. QHL1, a moderately halophilic bacterium isolated from Qinghai Lake.
Article in Journal of microbiology (Seoul, Korea), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 26 citations in OpenAlex.
- Genomic and functional characterization of Pseudosulfitobacter pseudonitzschiae BPC-C4-2: a growth-promoting symbiont in Antarctic Ulva communities.BMC genomics · 2026Article
- Comparative genomic analysis of Halomonas campaniensis wild-type and ultraviolet radiation-mutated strains reveal genomic differences associated with increased ectoine production.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Comparative Genome Analysis of a Novel Alkaliphilic Actinobacterial SpeciesPolish journal of microbiology · 2022Article
- Genome Sequence ofMicrobiology resource announcements · 2022Article
- Whole genome sequencing of the halophilic Halomonas qaidamensis XH36, a novel species strain with high ectoine production.Antonie van Leeuwenhoek · 2022Article
- Enhanced production of ectoine from methane using metabolically engineered Methylomicrobium alcaliphilum 20Z.Biotechnology for biofuels and bioproducts · 2022Article
- High ectoine production by an engineered Halomonas hydrothermalis Y2 in a reduced salinity medium.Microbial cell factories · 2019Article
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- New Alpiniamides FromFrontiers in microbiology · 2018Article
- Draft Genome Sequence of Halomonas elongata Strain K4, an Endophytic Growth-Promoting Bacterium Enhancing Salinity Tolerance In Planta.Genome announcements · 2016Article
- High production of ectoine from aspartate and glycerol by use of whole-cell biocatalysis in recombinant Escherichia coli.Microbial cell factories · 2015Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The moderately halophilic bacterium Halomonas sp. QHL1 was identified as a member of the genus Halomonas by 16S rRNA gene sequencing. HPLC analysis showed that strain QHL1 synthesizes ectoine in its cytoplasm. The genes involved in the ectoine biosynthesis pathway were identified on the chromosome in the order ectABC. Subsequently, the ectB gene from this strain was amplified by PCR, and the entire ectABC gene cluster (3,580 bp) was cloned using genome walking. Analysis showed that the ectA (579 bp), ectB (1269 bp), and ectC (390 bp) genes were organized in a single transcriptional unit and were predicted to encode three peptides of 21.2 kDa, 46.4 kDa, and 14.7 kDa, respectively. Two putative promoters, a δ(70)-dependent promoter and a δ(38)-controlled promoter, as well as several conserved motifs with unknown function were identified. Individual ectA, ectB, and ectC genes, and the entire ectABC gene cluster were inserted into the expression plasmid pET-28a(+) to generate the recombinant plasmids pET-28a(+)-ectA, pET-28a(+)-ectB, pET-28a(+)-ectC and pET-28a(+)-ectABC, respectively. Heterologous expression of these proteins in Escherichia coli BL21 (DE3) was confirmed by SDS-PAGE. The recombinant E. coli strain BL21 (pET-28a (+)-ectABC) displayed a higher salt tolerance than native E. coli cells but produced far less ectoine than the wild-type QHL1 strain.
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