ArticleParasites & vectors2013
Amoebic forms of Blastocystis spp. - evidence for a pathogenic role.
Article in Parasites & vectors, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Manipulation of host response and trajectory of parasite survival: A damage-response framework perspective.Virulence · 2026Review
- Site-Specific Incidence Rate of Blastocystis hominis and Its Association with Childhood Malnutrition: Findings from a Multi-Country Birth Cohort Study.The American journal of tropical medicine and hygiene · 2023Article
- Gut bacteria influence Blastocystis sp. phenotypes and may trigger pathogenicity.PLoS neglected tropical diseases · 2023Article
- Prevalence of Blastocystis sp. in Morocco: Comparative assessment of three diagnostic methods and characterization of parasite forms in Jones' culture medium.Parasite (Paris, France) · 2023Article
- Higher amoebic and metronidazole resistant forms of Blastocystis sp. seen in schizophrenic patients.Parasites & vectors · 2022Article
- Variations in active proteases ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Distinct Phenotypic Variation ofBiology · 2022Article
- Influence of Proton Pump Inhibitors and Histamine Receptor 2 Antagonists on Blastocystis ST3 and Selected Microorganisms of Intestinal Microbiota In Vitro.Clinical and translational gastroenterology · 2021Article
- Prevalence and subtypes of Blastocystis among migrant workers from different working sectors in Peninsular Malaysia.Parasitology research · 2020Article
- The influence of probiotic bacteria and human gut microorganisms causing opportunistic infections onGut pathogens · 2019Article
- Article
- Exacerbated symptoms in Blastocystis sp.-infected patients treated with metronidazole: two case studies.Parasitology research · 2018Article
- Blastocystis in Côte d'Ivoire: molecular identification and epidemiological data.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- A tale of two communities: intestinal polyparasitism among Orang Asli and Malay communities in rural Terengganu, Malaysia.Parasites & vectors · 2016Article
- Predominance of Blastocystis sp. Infection among School Children in Peninsular Malaysia.PloS one · 2016Article
- Increase number of mitochondrion-like organelle in symptomatic Blastocystis subtype 3 due to metronidazole treatment.Parasitology research · 2016Article
- Blastocystis specific serum immunoglobulin in patients with irritable bowel syndrome (IBS) versus healthy controls.Parasites & vectors · 2015Article
- Seeing the Whole Elephant: Imaging Flow Cytometry Reveals Extensive Morphological Diversity within Blastocystis Isolates.PloS one · 2015Article
- Blastocystis sp. in splenic cysts: causative agent or accidental association? A unique case report.Parasites & vectors · 2014Article
- Comparative Study of Wheatley's Trichrome Stain and In-vitro Culture against PCR Assay for the Diagnosis ofIranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBlastocystis spp. are one of the most prevalent parasites isolated from patients suffering from diarrhea, flatulence, constipation and vomiting. It's pathogenicity and pathophysiology remains controversial to date. Protease activity and amoebic forms have been reported previously in symptomatic isolates but there has been no conclusive evidence provided to correlate the protease activity and any specific life cycle stage of the parasite thus far.
methodsSymptomatic isolates with amoebic form were tested for protease activity and compared with symptomatic and asymptomatic isolates without amoebic form for 10 days culture period.
resultsThe present study demonstrates an elevated protease activity in cultures having a higher percentage of amoebic forms seen in symptomatic isolates. The growth curve demonstrated a significantly (p < 0.05) higher average number of parasite counts in asymptomatic compared to symptomatic isolates. Symptomatic isolates showed amoebic forms with percentages ranging from 5% to 17%. Elevated protease activity was demonstrated in isolates that had higher percentages of amoebic forms with intense bands at higher molecular weight proteases (60 - 100 kDa). As days of culture proceeded, the protease quantification also showed a steady increase.
conclusionThis study elucidates a correlation between protease activity and percentage of amoebic forms. The finding implies that these forms could play a role in exacerbation of intestinal symptoms during Blastocystis spp. infection.
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