ArticleTissue engineering. Part A2014
Micropatterned cell-cell interactions enable functional encapsulation of primary hepatocytes in hydrogel microtissues.
Article in Tissue engineering. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it, 134 citations in OpenAlex.
- A comprehensive review of advances in hepatocyte microencapsulation: selecting materials and preserving cell viability.Frontiers in immunology · 2024Pooled it
- A 3D In Vitro Model of the Human Hepatobiliary Junction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Functional material probes and advanced technologies in organ-on-a-chip characterization.Theranostics · 2026Review
- Organoid scaffold materials: research and application.Frontiers in bioengineering and biotechnology · 2025Review
- Hydrogel-Based Strategies for Liver Tissue Engineering.Chem & bio engineering · 2024Review
- A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications.Advanced materials technologies · 2024Article
- Fluid flow to mimic organ function in 3DAPL bioengineering · 2023Review
- Opportunities and considerations for studying liver disease with microphysiological systems on a chip.Lab on a chip · 2023Review
- Mechanobiology of cancer cell responsiveness to chemotherapy and immunotherapy: Mechanistic insights and biomaterial platforms.Advanced drug delivery reviews · 2023Review
- Nanofabrication Technologies to Control Cell and Tissue Function in Three-Dimension.Gels (Basel, Switzerland) · 2023Review
- Introducing CELLBLOKSIn vitro models · 2022Article
- Microfluidic Formulation of Topological Hydrogels for Microtissue Engineering.Chemical reviews · 2022Review
- Automated Analysis of Acetaminophen Toxicity on 3D HepaRG Cell Culture in Microbioreactor.Bioengineering (Basel, Switzerland) · 2022Article
- Towards organoid culture without Matrigel.Communications biology · 2021Review
- Controlled Apoptosis of Stromal Cells to Engineer Human Microlivers.Advanced functional materials · 2020Article
- Fibrillar Collagen Type I Participates in the Survival and Aggregation of Primary Hepatocytes Cultured on Soft Hydrogels.Biomimetics (Basel, Switzerland) · 2020Article
- Microscale Collagen and Fibroblast Interactions Enhance Primary Human Hepatocyte Functions in Three-Dimensional Models.Gene expression · 2020Article
- Microphysiological Systems: Design, Fabrication, and Applications.ACS biomaterials science & engineering · 2020Article
- Survival Rate of Cells Sent by a Low Mechanical Load Tube Pump: The "Ring Pump".Micromachines · 2020Article
- Liver Bioreactor Design Issues of Fluid Flow and Zonation, Fibrosis, and Mechanics: A Computational Perspective.Journal of functional biomaterials · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Drug-induced liver injury is a major cause of drug development failures and postmarket withdrawals. In vitro models that incorporate primary hepatocytes have been shown to be more predictive than model systems which rely on liver microsomes or hepatocellular carcinoma cell lines. Methods to phenotypically stabilize primary hepatocytes ex vivo often rely on mimicry of hepatic microenvironmental cues such as cell-cell interactions and cell-matrix interactions. In this work, we sought to incorporate phenotypically stable hepatocytes into three-dimensional (3D) microtissues, which, in turn, could be deployed in drug-screening platforms such as multiwell plates and diverse organ-on-a-chip devices. We first utilize micropatterning on collagen I to specify cell-cell interactions in two-dimensions, followed by collagenase digestion to produce well-controlled aggregates for 3D encapsulation in polyethylene glycol (PEG) diacrylate. Using this approach, we examined the influence of homotypic hepatocyte interactions and composition of the encapsulating hydrogel, and achieved the maintenance of liver-specific function for over 50 days. Optimally preaggregated structures were subsequently encapsulated using a microfluidic droplet-generator to produce 3D microtissues. Interactions of engineered hepatic microtissues with drugs was characterized by flow cytometry, and yielded both induction of P450 enzymes in response to prototypic small molecules and drug-drug interactions that give rise to hepatotoxicity. Collectively, this study establishes a pipeline for the manufacturing of 3D hepatic microtissues that exhibit stabilized liver-specific functions and can be incorporated into a wide array of emerging drug development platforms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.