Evidence map›Paper›PMID 24498468›Full record

ArticleThe Pan African medical journal2013

Overexpression of recombinant HIV-1 Subtype C Tat and Nef in a Salmonella vaccine vector.

Nyasha Chin'ombe, Maribanyana Lebeko, Mankgopo Kgatle

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Pan African medical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Nyasha Chin'ombeDivision of Medical Virology, Faculty of Health Sciences, University of Cape Town, Observatory 7925, Cape Town, South Africa ; Department of Medical Microbiology, College of Health Sciences, University of Zimbabwe, P O Box A178 Avondale, Harare, Zimbabwe.
Maribanyana LebekoDivision of Medical Virology, Faculty of Health Sciences, University of Cape Town, Observatory 7925, Cape Town, South Africa.
Mankgopo KgatleDivision of Medical Virology, Faculty of Health Sciences, University of Cape Town, Observatory 7925, Cape Town, South Africa.
University of Bahrain · BH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tat and Nef are very important regulatory proteins of HIV-1. They enhance viral replication and down-regulate expression of MHC Class I molecules, respectively. The antigens are now considered to be targets for HIV vaccine development. The expression of Tat and Nef in Salmonella vaccines has not previously been investigated. In this study, HIV-1 Subtype C tat and nef genes were cloned into an expression plasmid and their expression investigated in Salmonella. Very high-level expression of the two HIV-1 antigens was demonstrated in the recombinant Salmonella. The antigens were also successfully purified in bulk from the bacterium.Salmonella can therefore potentially be used to overexpress HIV-1 antigens and used as a possible delivery system in HIV-1 vaccine development.

Indexed as

Cloning, MolecularAIDS VaccinesGene Expression Regulation, BacterialGene Expression Regulation, ViralGenetic VectorsHIV-1HIV InfectionsHumansnef Gene Products, Human Immunodeficiency VirusSalmonella Vaccinestat Gene Products, Human Immunodeficiency VirusTransgenesAIDS Vaccinesnef Gene Products, Human Immunodeficiency Virusnef protein, Human immunodeficiency virus 1Salmonella Vaccinestat Gene Products, Human Immunodeficiency VirusHIVOverexpressionrecombinantregulatory proteinsSalmonellaviral replication

Identifiers

PMID24498468
PMCPMC3909692
OpenAlexW2028149389

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.