ArticlePloS one2014
Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.
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Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Advances in the research of the role of macrophage/microglia polarization-mediated inflammatory response in spinal cord injury.Frontiers in immunology · 2022Pooled it
- Bone Marrow-Derived Mesenchymal Stem Cells Alleviate Cutaneous Leishmaniasis by Promoting M2 Macrophage Polarization and Skin Tissue Repair in a Murine Model.Biomolecules · 2026Article
- STINGing Cancer: Development, Clinical Application, and Targeted Delivery of STING Agonists.International journal of molecular sciences · 2025Review
- Macrophages: Subtypes, Distribution, Polarization, Immunomodulatory Functions, and Therapeutics.MedComm · 2025Review
- Sarcoidosis and Cancer: The Role of the Granulomatous Reaction as a Double-Edged Sword.Journal of clinical medicine · 2024Article
- Role of microglia/macrophage polarisation in intraocular diseases (Review).International journal of molecular medicine · 2024Review
- Automated single-cell proteomics providing sufficient proteome depth to study complex biology beyond cell type classifications.bioRxiv : the preprint server for biology · 2024Article
- Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding.Advanced drug delivery reviews · 2023Review
- Interaction between Porcine Alveolar Macrophage-Tang Cells and Streptococcus suis Strains of Different Virulence: Phagocytosis and ApoptosisMicroorganisms · 2023Article
- Synovial Fluid in Knee Osteoarthritis Extends Proinflammatory Niche for Macrophage Polarization.Cells · 2022Article
- Distinct non-coding RNA cargo of extracellular vesicles from M1 and M2 human primary macrophages.Journal of extracellular vesicles · 2022Article
- Exosomes derived from bone marrow mesenchymal stem cells inhibit neuroinflammation after traumatic brain injury.Neural regeneration research · 2022Article
- The pathogenesis of cryptoglandular anal fistula: New insight into the immunological profile.Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland · 2022Article
- PCSK9 Confers Inflammatory Properties to Extracellular Vesicles Released by Vascular Smooth Muscle Cells.International journal of molecular sciences · 2022Article
- Insights into innovative therapeutics for drug-resistant tuberculosis: Host-directed therapy and autophagy inducing modified nanoparticles.International journal of pharmaceutics · 2022Article
- Methodological Approaches To Assess Innate Immunity and Innate Memory in Marine Invertebrates and Humans.Frontiers in toxicology · 2022Review
- Tendon healing is adversely affected by low-grade inflammation.Journal of orthopaedic surgery and research · 2021Review
- In Vitro and In Vivo Models to Assess the Immune-Related Effects of Nanomaterials.International journal of environmental research and public health · 2021Review
- Macrophage Identification In Situ.Biomedicines · 2021Review
- Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monocytes/macrophages are key players in all phases of physiological and pathological inflammation. To understanding the regulation of macrophage functional differentiation during inflammation, we designed an in vitro model that recapitulates the different phases of the reaction (recruitment, initiation, development, and resolution), based on human primary blood monocytes exposed to sequential changes in microenvironmental conditions. All reaction phases were profiled by transcriptomic microarray analysis. Distinct clusters of genes were identified that are differentially regulated through the different phases of inflammation. The gene sets defined by GSEA analysis revealed that the inflammatory phase was enriched in inflammatory pathways, while the resolution phase comprised pathways related to metabolism and gene rearrangement. By comparing gene clusters differentially expressed in monocytes vs. M1 and vs. M2 macrophages extracted from an in-house created meta-database, it was shown that cells in the model resemble M1 during the inflammatory phase and M2 during resolution. The validation of inflammatory and transcriptional factors by qPCR and ELISA confirmed the transcriptomic profiles in the different phases of inflammation. The accurate description of the development of the human inflammatory reaction provided by this in vitro kinetic model can help in identifying regulatory mechanisms in physiological conditions and during pathological derangements.
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Registered trials
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