ArticlePloS one2014
Use of self-complementary adeno-associated virus serotype 2 as a tracer for labeling axons: implications for axon regeneration.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Compensatory adaptation of parallel motor pathways promotes skilled forelimb recovery after spinal cord injury.iScience · 2024Article
- STAT3 protects dopaminergic neurons against degeneration in animal model of Parkinson's disease.Brain research · 2024Article
- Generation of direct current electrical fields as regenerative therapy for spinal cord injury: A review.APL bioengineering · 2023Review
- Article
- Axotomy Induces Drp1-Dependent Fragmentation of Axonal Mitochondria.Frontiers in molecular neuroscience · 2021Article
- Upregulating Lin28a Promotes Axon Regeneration in Adult Mice with Optic Nerve and Spinal Cord Injury.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Article
- scAAV2-Mediated C3 Transferase Gene Therapy in a Rat Model with Retinal Ischemia/Reperfusion Injuries.Molecular therapy. Methods & clinical development · 2020Article
- AAV2-BDNF promotes respiratory axon plasticity and recovery of diaphragm function following spinal cord injury.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Partial Reconstruction of the Nigrostriatal Circuit along a Preformed Molecular Guidance Pathway.Molecular therapy. Methods & clinical development · 2019Article
- Promoting Axon Regeneration in Adult CNS by Targeting Liver Kinase B1.Molecular therapy : the journal of the American Society of Gene Therapy · 2019Article
- Retrogradely Transportable Lentivirus Tracers for Mapping Spinal Cord Locomotor Circuits.Frontiers in neural circuits · 2018Article
- Synaptic Phospholipid Signaling Modulates Axon Outgrowth via Glutamate-dependent Ca2+-mediated Molecular Pathways.Cerebral cortex (New York, N.Y. : 1991) · 2017Article
- NT-3 promotes proprioceptive axon regeneration when combined with activation of the mTor intrinsic growth pathway but not with reduction of myelin extrinsic inhibitors.Experimental neurology · 2016Article
- rAAV/ABAD-DP-6His attenuates oxidative stress-induced injury of PC12 cells.Neural regeneration research · 2014Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Various types of tracers are available for use in axon regeneration, but they require an extra operational tracer injection, time-consuming immunohistochemical analysis and cause non-specific labeling. Considerable efforts over the past years have explored other methodologies, especially the use of viral vectors, to investigate axon regeneration after injury. Recent studies have demonstrated that self-complementary Adeno-Associated Virus (scAAV) induced a high transduction efficiency and faster expression of transgenes. Here, we describe for the first time the use of scAAV2-GFP to label long-projection axons in the corticospinal tract (CST), rubrospinal tract (RST) and the central axons of dorsal root ganglion (DRG) in the normal and lesioned animal models. We found that scAAV2-GFP could efficiently transduce neurons in the sensorimotor cortex, red nucleus and DRG. Strong GFP expression could be transported anterogradely along the axon to label the numerous axon fibers from CST, RST and central axons of DRG separately. Comparison of the scAAV2 vector with single-stranded (ss) AAV2 vector in co-labeled sections showed that the scAAV2 vector induced a faster and stronger transgene expression than the ssAAV2 vector in DRG neurons and their axons. In both spinal cord lesion and dorsal root crush injury models, scAAV-GFP could efficiently label the lesioned and regenerated axons around the lesion cavity and the dorsal root entry zone (DREZ) respectively. Further, scAAV2-GFP vector could be combined with traditional tracer to specifically label sensory and motor axons after spinal cord lesion. Thus, we show that using scAAV2-GFP as a tracer is a more effective and efficient way to study axon regeneration following injury.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.