ArticlePloS one2014
Validation of reference genes aiming accurate normalization of qRT-PCR data in Dendrocalamus latiflorus Munro.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Genome-Wide Identification and Role of theInternational journal of molecular sciences · 2024Article
- Selection and Validation of Reference Genes inPlants (Basel, Switzerland) · 2024Article
- Expression of Iron Metabolism Genes Is Potentially Regulated by DOF Transcription Factors inInternational journal of molecular sciences · 2024Article
- Identification and characterization of the cupin_1 domain-containing proteins in ma bamboo (Frontiers in plant science · 2023Article
- TCP Transcription Factors Involved in Shoot Development of Ma Bamboo (Frontiers in plant science · 2022Article
- Identification and selection of reference genes for gene expression analysis by quantitative real-time PCR in Suaeda glauca's response to salinity.Scientific reports · 2021Article
- Integrated Transcriptome Analysis and Single-Base Resolution Methylomes of Watermelon (Frontiers in plant science · 2021Article
- Complementary Transcriptome and Proteome Analyses Provide Insight into the Floral Transition in Bamboo (International journal of molecular sciences · 2020Article
- Evaluation of reference genes at different developmental stages for quantitative real-time PCR in Aedes aegypti.Scientific reports · 2017Article
- An Efficient Plant Regeneration and Transformation System of Ma Bamboo (Frontiers in plant science · 2017Article
- Selection of reliable reference genes for normalization of quantitative RT-PCR from different developmental stages and tissues in amphioxus.Scientific reports · 2016Article
- Selection of Reliable Reference Genes for Gene Expression Studies onFrontiers in plant science · 2016Article
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Abstract
backgroundDendrocalamus latiflorus Munro distributes widely in subtropical areas and plays vital roles as valuable natural resources. The transcriptome sequencing for D. latiflorus Munro has been performed and numerous genes especially those predicted to be unique to D. latiflorus Munro were revealed. qRT-PCR has become a feasible approach to uncover gene expression profiling, and the accuracy and reliability of the results obtained depends upon the proper selection of stable reference genes for accurate normalization. Therefore, a set of suitable internal controls should be validated for D. latiflorus Munro.
resultsIn this report, twelve candidate reference genes were selected and the assessment of gene expression stability was performed in ten tissue samples and four leaf samples from seedlings and anther-regenerated plants of different ploidy. The PCR amplification efficiency was estimated, and the candidate genes were ranked according to their expression stability using three software packages: geNorm, NormFinder and Bestkeeper. GAPDH and EF1α were characterized to be the most stable genes among different tissues or in all the sample pools, while CYP showed low expression stability. RPL3 had the optimal performance among four leaf samples. The application of verified reference genes was illustrated by analyzing ferritin and laccase expression profiles among different experimental sets. The analysis revealed the biological variation in ferritin and laccase transcript expression among the tissues studied and the individual plants.
conclusionsgeNorm, NormFinder, and BestKeeper analyses recommended different suitable reference gene(s) for normalization according to the experimental sets. GAPDH and EF1α had the highest expression stability across different tissues and RPL3 for the other sample set. This study emphasizes the importance of validating superior reference genes for qRT-PCR analysis to accurately normalize gene expression of D. latiflorus Munro.
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