ArticlePloS one2014
Ultra deep sequencing of Listeria monocytogenes sRNA transcriptome revealed new antisense RNAs.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Comprehensive insights into the metabolism characteristics of small RNA Qrr4 in Vibrio alginolyticus.Applied microbiology and biotechnology · 2023Article
- Comparative analysis of Listeria monocytogenes plasmid transcriptomes reveals common and plasmid-specific gene expression patterns and high expression of noncoding RNAs.MicrobiologyOpen · 2022Article
- RNA-Mediated Control inFrontiers in microbiology · 2021Review
- Listeria monocytogenes: towards a complete picture of its physiology and pathogenesis.Nature reviews. Microbiology · 2018Review
- Recent advances in understandingF1000Research · 2017Review
- Article
- Two novel members of the LhrC family of small RNAs in Listeria monocytogenes with overlapping regulatory functions but distinctive expression profiles.RNA biology · 2016Article
- The Mechanisms of Virulence Regulation by Small Noncoding RNAs in Low GC Gram-Positive Pathogens.International journal of molecular sciences · 2015Review
- Survival of Listeria monocytogenes in Soil Requires AgrA-Mediated Regulation.Applied and environmental microbiology · 2015Article
- Quantitative bacterial transcriptomics with RNA-seq.Current opinion in microbiology · 2015Review
- A detailed view of the intracellular transcriptome of Listeria monocytogenes in murine macrophages using RNA-seq.Frontiers in microbiology · 2015Article
- Strand-specific community RNA-seq reveals prevalent and dynamic antisense transcription in human gut microbiota.Frontiers in microbiology · 2015Article
- The multicopy sRNA LhrC controls expression of the oligopeptide-binding protein OppA in Listeria monocytogenes.RNA biology · 2015Article
- Unprecedented high-resolution view of bacterial operon architecture revealed by RNA sequencing.mBio · 2014Article
- Non-coding RNA regulation in pathogenic bacteria located inside eukaryotic cells.Frontiers in cellular and infection microbiology · 2014Review
- Current status of antisense RNA-mediated gene regulation in Listeria monocytogenes.Frontiers in cellular and infection microbiology · 2014Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes, a gram-positive pathogen, and causative agent of listeriosis, has become a widely used model organism for intracellular infections. Recent studies have identified small non-coding RNAs (sRNAs) as important factors for regulating gene expression and pathogenicity of L. monocytogenes. Increased speed and reduced costs of high throughput sequencing (HTS) techniques have made RNA sequencing (RNA-Seq) the state-of-the-art method to study bacterial transcriptomes. We created a large transcriptome dataset of L. monocytogenes containing a total of 21 million reads, using the SOLiD sequencing technology. The dataset contained cDNA sequences generated from L. monocytogenes RNA collected under intracellular and extracellular condition and additionally was size fractioned into three different size ranges from <40 nt, 40-150 nt and >150 nt. We report here, the identification of nine new sRNAs candidates of L. monocytogenes and a reevaluation of known sRNAs of L. monocytogenes EGD-e. Automatic comparison to known sRNAs revealed a high recovery rate of 55%, which was increased to 90% by manual revision of the data. Moreover, thorough classification of known sRNAs shed further light on their possible biological functions. Interestingly among the newly identified sRNA candidates are antisense RNAs (asRNAs) associated to the housekeeping genes purA, fumC and pgi and potentially their regulation, emphasizing the significance of sRNAs for metabolic adaptation in L. monocytogenes.
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