ArticlePloS one2014
Indian-ink perfusion based method for reconstructing continuous vascular networks in whole mouse brain.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
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- On the Fila Olfactoria and the Cribriform Region of the Crocodylia.Journal of morphology · 2025Article
- Paclitaxel alters the microvascular network in the central and peripheral nervous system of rats with chemotherapy-induced painful peripheral neuropathy.Journal of the peripheral nervous system : JPNS · 2024Article
- Cortical spreading depolarization-induced constriction of penetrating arteries can cause watershed ischemia: A potential mechanism for white matter lesions.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023Article
- A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution.Cell reports methods · 2023Article
- Structural and functional imaging of brains.Science China. Chemistry · 2023Review
- A modified mouse model of haemorrhagic transformation associated with tPA administration after thromboembolic stroke.Heliyon · 2023Article
- Advances in studying whole mouse brain vasculature using high-resolution 3D light microscopy imaging.Neurophotonics · 2022Review
- VascuViz: a multimodality and multiscale imaging and visualization pipeline for vascular systems biology.Nature methods · 2022Article
- NmFGF1-Regulated Glucolipid Metabolism and Angiogenesis Improves Functional Recovery in a Mouse Model of Diabetic Stroke and ActsFrontiers in pharmacology · 2021Article
- Advancing brain barriers RNA sequencing: guidelines from experimental design to publication.Fluids and barriers of the CNS · 2020Review
- Visualization and molecular characterization of whole-brain vascular networks with capillary resolution.Nature communications · 2020Article
- Sural Nerve Perfusion in Mice.Frontiers in neuroscience · 2020Article
- Detection of Cerebrovascular Loss in the Normal Aging C57BL/6 Mouse Brain UsingFrontiers in aging neuroscience · 2020Article
- The cerebral angiome: High resolution MicroCT imaging of the whole brain cerebrovasculature in female and male mice.NeuroImage · 2019Article
- Review of micro-optical sectioning tomography (MOST): technology and applications for whole-brain optical imaging [Invited].Biomedical optics express · 2019Article
- Cerebral perfusion and compensatory blood supply in patients with recent small subcortical infarcts.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2019Article
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8 authors.
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Abstract
The topology of the cerebral vasculature, which is the energy transport corridor of the brain, can be used to study cerebral circulatory pathways. Limited by the restrictions of the vascular markers and imaging methods, studies on cerebral vascular structure now mainly focus on either observation of the macro vessels in a whole brain or imaging of the micro vessels in a small region. Simultaneous vascular studies of arteries, veins and capillaries have not been achieved in the whole brain of mammals. Here, we have combined the improved gelatin-Indian ink vessel perfusion process with Micro-Optical Sectioning Tomography for imaging the vessel network of an entire mouse brain. With 17 days of work, an integral dataset for the entire cerebral vessels was acquired. The voxel resolution is 0.35×0.4×2.0 µm(3) for the whole brain. Besides the observations of fine and complex vascular networks in the reconstructed slices and entire brain views, a representative continuous vascular tracking has been demonstrated in the deep thalamus. This study provided an effective method for studying the entire macro and micro vascular networks of mouse brain simultaneously.
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