Trial reportPloS one2014
Circulating contact-pathway-activating microparticles together with factors IXa and XIa induce spontaneous clotting in plasma of hematology and cardiologic patients.
Trial report in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 63 citations in OpenAlex.
- Heparin and Direct Oral Anticoagulants have Different Effects on the Phases of Activation and Spatial Spread of Blood Coagulation.Thrombosis and haemostasis · 2026Article
- Fibrinography and thrombography (thrombodynamics-4D) in atrial fibrillation assessment of direct oral anticoagulants in geriatrics patients aged 80 years and older receiving direct oral anticoagulant therapy.Research and practice in thrombosis and haemostasis · 2025Article
- Comparison of standard and global hemostasis assays in cord and peripheral blood of newborns.Pediatric research · 2025Article
- Thrombogenic potential of picomolar coagulation factor XIa is mediated by thrombin wave propagation.Blood advances · 2023Article
- Integral assays of hemostasis in hospitalized patients with COVID-19 on admission and during heparin thromboprophylaxis.PloS one · 2023Article
- Ozonation of Whole Blood Results in an Increased Release of Microparticles from Blood Cells.Biomolecules · 2022Article
- Production of erythrocyte microparticles in a sub-hemolytic environment.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2021Article
- Redistribution of TPA Fluxes in the Presence of PAI-1 Regulates Spatial Thrombolysis.Biophysical journal · 2020Article
- Article
- Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential.Journal of extracellular vesicles · 2019Article
- An enhanced clot growth rate before in vitro fertilization decreases the probability of pregnancy.PloS one · 2019Observational
- Impaired platelet activity and hypercoagulation in healthy term and moderately preterm newborns during the early neonatal period.Pediatric research · 2019Article
- Article
- Thrombodynamics-A new global hemostasis assay for heparin monitoring in patients under the anticoagulant treatment.PloS one · 2018Article
- Article
- Random Forests Are Able to Identify Differences in Clotting Dynamics from Kinetic Models of Thrombin Generation.PloS one · 2016Article
- Procoagulant impact of the plasmapheresis procedure on coagulation state of collected plasma.Blood transfusion = Trasfusione del sangue · 2015Article
- Global assays of hemostasis in the diagnostics of hypercoagulation and evaluation of thrombosis risk.Thrombosis journal · 2015Article
- Article
- Platelet surface-associated activation and secretion-mediated inhibition of coagulation factor XII.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
BACKGROUND AND
objectiveUsing an in vitro experimental model of immobilized tissue factor-initiated clot growth in platelet-free plasma (thrombodynamics), we observed formation of activator-independent isolated spontaneous clots (SC) throughout the plasma volume in patients with cardiac infarction, acute leukemia, hemolytic anemia, and some other disorders. The aim of this work was to characterize this phenomenon and to identify the mechanisms of SC formation. METHODS AND
resultsTissue factor inhibitor (VIIai) prevented SC in only 2 out of 23 patient plasma samples. Specific inhibitors of factors IXa and XIa were efficient in all 8 cases that we tested. Also, only factors IXa and XIa added to normal donors' plasma induced SC formations from isolated centers, in a pattern similar to that in patients' plasma. In contrast, factors VIIa, Va, tissue factor induced uniform plasma clotting. SC disappeared after high-speed centrifugation. However, phospholipid supplementation of centrifuged plasma returned them at least partially in 5 out of 22 patients' plasmas, indicating some other role of microparticles than providing phospholipid surface. Circulating procoagulant microparticles isolated from plasma directly activated factor XII in buffer and in diluted plasma. Flow cytometry revealed an increase in procoagulant microparticles in patients' plasmas with SC.
conclusionOur data suggest that combination of circulating active factors (specifically, factors IXa and XIa) with circulating procoagulant and contact-pathway-activating microparticles is the predominant mechanism causing spontaneous clotting in patient plasma.
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