ArticlePloS one2014
Transcriptome analysis in venom gland of the predatory giant ant Dinoponera quadriceps: insights into the polypeptide toxin arsenal of hymenopterans.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed.
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- A rare, evolutionarily conserved venom protein benefits endoparasitism across parasitoids.Cell genomics · 2025Article
- The Helix Ring Peptide UMembranes · 2024Article
- DeTox: a pipeline for the detection of toxins in venomous organisms.Briefings in bioinformatics · 2024Article
- Unraveling the Role of Antimicrobial Peptides in Insects.International journal of molecular sciences · 2023Review
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- A combined protein toxin screening based on the transcriptome and proteome of Solenopsis invicta.Proteome science · 2022Article
- Profiling hymenopteran venom toxins: Protein families, structural landscape, biological activities, and pharmacological benefits.Toxicon: X · 2022Article
- Transcriptomic Analysis of the Venom Gland and Enzymatic Characterization of the Venom ofToxins · 2022Article
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- Multipurpose peptides: The venoms of Amazonian stinging ants contain anthelmintic ponericins with diverse predatory and defensive activities.Biochemical pharmacology · 2021Article
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- Composition and Acute Inflammatory Response fromToxins · 2021Article
- An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components.Scientific reports · 2021Article
- Multiple transcriptome mining coupled with tissue specific molecular cloning and mass spectrometry provide insights into agatoxin-like peptide conservation in decapod crustaceans.General and comparative endocrinology · 2020Article
- Antifungal In Vitro Activity of Pilosulin- and Ponericin-Like Peptides from the Giant AntAntibiotics (Basel, Switzerland) · 2020Article
- An Integrated Proteomic and Transcriptomic Analysis Reveals the Venom Complexity of the Bullet AntToxins · 2020Article
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- Article
- Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDinoponera quadriceps is a predatory giant ant that inhabits the Neotropical region and subdues its prey (insects) with stings that deliver a toxic cocktail of molecules. Human accidents occasionally occur and cause local pain and systemic symptoms. A comprehensive study of the D. quadriceps venom gland transcriptome is required to advance our knowledge about the toxin repertoire of the giant ant venom and to understand the physiopathological basis of Hymenoptera envenomation.
resultsWe conducted a transcriptome analysis of a cDNA library from the D. quadriceps venom gland with Sanger sequencing in combination with whole-transcriptome shotgun deep sequencing. From the cDNA library, a total of 420 independent clones were analyzed. Although the proportion of dinoponeratoxin isoform precursors was high, the first giant ant venom inhibitor cysteine-knot (ICK) toxin was found. The deep next generation sequencing yielded a total of 2,514,767 raw reads that were assembled into 18,546 contigs. A BLAST search of the assembled contigs against non-redundant and Swiss-Prot databases showed that 6,463 contigs corresponded to BLASTx hits and indicated an interesting diversity of transcripts related to venom gene expression. The majority of these venom-related sequences code for a major polypeptide core, which comprises venom allergens, lethal-like proteins and esterases, and a minor peptide framework composed of inter-specific structurally conserved cysteine-rich toxins. Both the cDNA library and deep sequencing yielded large proportions of contigs that showed no similarities with known sequences.
conclusionsTo our knowledge, this is the first report of the venom gland transcriptome of the New World giant ant D. quadriceps. The glandular venom system was dissected, and the toxin arsenal was revealed; this process brought to light novel sequences that included an ICK-folded toxins, allergen proteins, esterases (phospholipases and carboxylesterases), and lethal-like toxins. These findings contribute to the understanding of the ecology, behavior and venomics of hymenopterans.
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