ArticlePloS one2014
SinR controls enterotoxin expression in Bacillus thuringiensis biofilms.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 96 citations in OpenAlex.
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- The major toxins of Bacillus cereus - Current knowledge on distribution, regulation and mechanism of action.EXCLI journal · 2026Review
- Integrated Biofilm Modification and Transcriptional Analysis for Improving Fengycin Production in Bacillus amyloliquefaciens.Probiotics and antimicrobial proteins · 2025Article
- Gene expression dynamics inFrontiers in microbiology · 2025Article
- A novel regulator CdsR negatively regulates cell motility in Bacillus thuringiensis.Scientific reports · 2024Article
- Genomic analysis and assessment of pathogenic (toxicogenic) potential of Staphylococcus haemolyticus and Bacillus paranthracis consortia isolated from bovine mastitis in Russia.Scientific reports · 2023Article
- A review on surfactin: molecular regulation of biosynthesis.Archives of microbiology · 2023Review
- Virulence-related genotypic differences amongFrontiers in cellular and infection microbiology · 2023Article
- Evaluation of the biofilm-forming ability and molecular characterization of dairyFrontiers in cellular and infection microbiology · 2023Article
- Review
- The Physiological Functions of AbrB on Sporulation, Biofilm Formation and Carbon Source Utilization inBioengineering (Basel, Switzerland) · 2022Article
- Expression of the Bacillus thuringiensisMicrobiology spectrum · 2022Article
- Adaptation of Bacillus thuringiensis to Plant Colonization Affects Differentiation and Toxicity.mSystems · 2021Article
- Transition Phase Regulator AbrB Positively Regulates theMicrobiology spectrum · 2021Article
- Sporulation is dispensable for the vegetable-associated life cycle of the human pathogen Bacillus cereus.Microbial biotechnology · 2021Article
- TheMicrobiology (Reading, England) · 2021Article
- Generation of Genetic Tools for Gauging Multiple-Gene Expression at the Single-Cell Level.Applied and environmental microbiology · 2021Article
- Massive Integration of Planktonic Cells within a Developing Biofilm.Microorganisms · 2021Article
- The Food Poisoning Toxins ofToxins · 2021Review
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The entomopathogen Bacillus thuringiensis produces dense biofilms under various conditions. Here, we report that the transition phase regulators Spo0A, AbrB and SinR control biofilm formation and swimming motility in B. thuringiensis, just as they control biofilm formation and swarming motility in the closely related saprophyte species B. subtilis. However, microarray analysis indicated that in B. thuringiensis, in contrast to B. subtilis, SinR does not control an eps operon involved in exopolysaccharides production, but regulates genes involved in the biosynthesis of the lipopeptide kurstakin. This lipopeptide is required for biofilm formation and was previously shown to be important for survival in the host cadaver (necrotrophism). Microarray analysis also revealed that the SinR regulon contains genes coding for the Hbl enterotoxin. Transcriptional fusion assays, Western blots and hemolysis assays confirmed that SinR controls Hbl expression, together with PlcR, the main virulence regulator in B. thuringiensis. We show that Hbl is expressed in a sustained way in a small subpopulation of the biofilm, whereas almost all the planktonic population transiently expresses Hbl. The gene coding for SinI, an antagonist of SinR, is expressed in the same biofilm subpopulation as hbl, suggesting that hbl transcription heterogeneity is SinI-dependent. B. thuringiensis and B. cereus are enteric bacteria which possibly form biofilms lining the host intestinal epithelium. Toxins produced in biofilms could therefore be delivered directly to the target tissue.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.