ArticlePloS one2014
AtWuschel promotes formation of the embryogenic callus in Gossypium hirsutum.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 76 citations in OpenAlex.
- WUSCHEL Transcription Factor: From Stem Cell Maintenance to Crop Improvement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- Integrating meristem initiation and floral fate control: towardFrontiers in plant science · 2026Review
- Efficient and versatile rapeseed transformation for new breeding technologies.The Plant journal : for cell and molecular biology · 2025Article
- Spatiotemporal transcriptome and metabolome landscapes of cotton somatic embryos.Nature communications · 2025Article
- Review
- Functional Mechanisms and the Application of Developmental Regulators for Improving Genetic Transformation in Plants.Plants (Basel, Switzerland) · 2024Review
- Appreciating animal induced pluripotent stem cells to shape plant cell reprogramming strategies.Journal of experimental botany · 2024Review
- Application of Developmental Regulators for Enhancing Plant Regeneration and Genetic Transformation.Plants (Basel, Switzerland) · 2024Review
- Epigenetic modifications and miRNAs determine the transition of somatic cells into somatic embryos.Plant cell reports · 2023Review
- Comparative transcriptome analysis of high- and low-embryogenic Hevea brasiliensis genotypes reveals involvement of phytohormones in somatic embryogenesis.BMC plant biology · 2023Article
- Identification and Evolutionary Analysis of Cotton (International journal of molecular sciences · 2023Article
- Advances in Somatic Embryogenesis of Banana.International journal of molecular sciences · 2023Review
- Changes and transcriptome regulation of endogenous hormones during somatic embryogenesis inFrontiers in plant science · 2023Article
- Induction of Somatic Embryogenesis in Plants: Different Players and Focus on WUSCHEL and WUS-RELATED HOMEOBOX (WOX) Transcription Factors.International journal of molecular sciences · 2022Review
- Genetic and epigenetic modes of the regulation of somatic embryogenesis: a review.Biologia futura · 2022Review
- miR172 RegulatesCells · 2022Article
- Transduction of Signals during Somatic Embryogenesis.Plants (Basel, Switzerland) · 2022Review
- Constitutive Overexpression of a ConiferFrontiers in plant science · 2022Article
- Uniconazole Augments Abscisic Acid in Promoting Somatic Embryogenesis in Cotton (Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Upland cotton (Gossypium hirsutum) is one of the most recalcitrant species for in vitro plant regeneration through somatic embryogenesis. Callus from only a few cultivars can produce embryogenic callus (EC), but the mechanism is not well elucidated. Here we screened a cultivar, CRI24, with high efficiency of EC produce. The expression of genes relevant to EC production was analyzed between the materials easy to or difficult to produce EC. Quantitative PCR showed that CRI24, which had a 100% EC differentiation rate, had the highest expression of the genes GhLEC1, GhLEC2, and GhFUS3. Three other cultivars, CRI12, CRI41, and Lu28 that formed few ECs expressed these genes only at low levels. Each of the genes involved in auxin transport (GhPIN7) and signaling (GhSHY2) was most highly expressed in CRI24, with low levels in the other three cultivars. WUSCHEL (WUS) is a homeodomain transcription factor that promotes the vegetative-to-embryogenic transition. We thus obtained the calli that ectopically expressed Arabidopsis thaliana Wus (AtWus) in G. hirsutum cultivar CRI12, with a consequent increase of 47.75% in EC differentiation rate compared with 0.61% for the control. Ectopic expression of AtWus in CRI12 resulted in upregulation of GhPIN7, GhSHY2, GhLEC1, GhLEC2, and GhFUS3. AtWus may therefore increase the differentiation potential of cotton callus by triggering the auxin transport and signaling pathways.
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