ArticlePloS one2014
Transcriptional profiling of ParA and ParB mutants in actively dividing cells of an opportunistic human pathogen Pseudomonas aeruginosa.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 34 citations in OpenAlex.
- DnaA binding to oriCII regulates transcription from Pseudomonas aeruginosa gidAB-parAB locus linked to the chromosome segregation and streptomycin resistance.Scientific reports · 2026Article
- RpoN mediates biofilm formation by directly controllingBiofilm · 2025Article
- Structure and functions of a multireplicon genome of Antarctic Psychrobacter sp. ANT_H3: characterization of the genetic modules suitable for the construction of the plasmid-vectors for cold-active bacteria.Journal of applied genetics · 2023Article
- Robust ParB Binding to Half-International journal of molecular sciences · 2023Article
- Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa.Microbiology spectrum · 2023Article
- Functional Characterization of TetR-like Transcriptional Regulator PA3973 fromInternational journal of molecular sciences · 2022Article
- Immunometabolism in biofilm infection: lessons from cancer.Molecular medicine (Cambridge, Mass.) · 2022Review
- The Lrp/AsnC-Type Regulator PA2577 Controls the EamA-like Transporter GeneInternational journal of molecular sciences · 2021Article
- The AraC-Type Transcriptional Regulator GliR (PA3027) Activates Genes of Glycerolipid Metabolism inInternational journal of molecular sciences · 2021Article
- The MarR-Type Regulator PA3458 Is Involved in Osmoadaptation Control inInternational journal of molecular sciences · 2021Article
- Identification and Characterization of the First Virulent Phages, Including a Novel Jumbo Virus, InfectingInternational journal of molecular sciences · 2020Article
- Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes.Microorganisms · 2020Review
- Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element.BMC genomics · 2020Article
- Chromosome Segregation Proteins as Coordinators of Cell Cycle in Response to Environmental Conditions.Frontiers in microbiology · 2020Review
- Diversity and Horizontal Transfer of AntarcticGenes · 2019Article
- Benefits and Drawbacks of Harboring Plasmid pP32BP2, Identified in Arctic Psychrophilic BacteriumInternational journal of molecular sciences · 2019Article
- Random Chromosome Partitioning in the Polyploid BacteriumG3 (Bethesda, Md.) · 2019Article
- Helicobacter pylori Biofilm Formation and Its Potential Role in Pathogenesis.Microbiology and molecular biology reviews : MMBR · 2018Review
- Pseudomonas aeruginosa partitioning protein ParB acts as a nucleoid-associated protein binding to multiple copies of a parS-related motif.Nucleic acids research · 2018Article
- Plasmids of PsychrotolerantFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate chromosome segregation to progeny cells is a fundamental process ensuring proper inheritance of genetic material. In bacteria with simple cell cycle, chromosome segregation follows replication initiation since duplicated oriC domains start segregating to opposite halves of the cell soon after they are made. ParA and ParB proteins together with specific DNA sequences are parts of the segregation machinery. ParA and ParB proteins in Pseudomonas aeruginosa are important for optimal growth, nucleoid segregation, cell division and motility. Comparative transcriptome analysis of parA null and parB null mutants versus parental P. aeruginosa PAO1161 strain demonstrated global changes in gene expression pattern in logarithmically growing planktonic cultures. The set of genes similarly affected in both mutant strains is designated Par regulon and comprises 536 genes. The Par regulon includes genes controlled by two sigma factors (RpoN and PvdS) as well as known and putative transcriptional regulators. In the absence of Par proteins, a large number of genes from RpoS regulon is induced, reflecting the need for slowing down the cell growth rate and decelerating the metabolic processes. Changes in the expression profiles of genes involved in c-di-GMP turnover point out the role of this effector in such signal transmission. Microarray data for chosen genes were confirmed by RT-qPCR analysis. The promoter regions of selected genes were cloned upstream of the promoter-less lacZ gene and analyzed in the heterologous host E. coliΔlac. Regulation by ParA and ParB of P. aeruginosa was confirmed for some of the tested promoters. Our data demonstrate that ParA and ParB besides their role in accurate chromosome segregation may act as modulators of genes expression. Directly or indirectly, Par proteins are part of the wider regulatory network in P. aeruginosa linking the process of chromosome segregation with the cell growth, division and motility.
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