ArticlePloS one2014
Staphylococcus aureus interaction with phospholipid vesicles--a new method to accurately determine accessory gene regulator (agr) activity.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Phosphate sensing by PhoPR regulates the cytotoxicity ofMicrobiology (Reading, England) · 2025Article
- Bacterial Toxin-Triggered Release of Antibiotics from Capsosomes Protects a Fly Model from Lethal Methicillin-Resistant Staphylococcus aureus (MRSA) Infection.Advanced healthcare materials · 2022Article
- Identification of small molecules that strongly inhibit bacterial quorum sensing using a high-throughput lipid vesicle lysis assay.Cell chemical biology · 2022Article
- Multiplexed detection and differentiation of bacterial enzymes and bacteria by color-encoded sensor hydrogels.Bioactive materials · 2021Article
- Article
- Liquid Crystal Emulsions That Intercept and Report on Bacterial Quorum Sensing.ACS applied materials & interfaces · 2020Article
- Bacteriophage-Antibiotic Combination Strategy: an Alternative against Methicillin-Resistant Phenotypes of Staphylococcus aureus.Antimicrobial agents and chemotherapy · 2020Article
- Cigarette smoke exposure redirects Staphylococcus aureus to a virulence profile associated with persistent infection.Scientific reports · 2019Article
- [The inhibition of accessory gene regulator C specific binding peptides on biofilm formation ofZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2019Article
- Disease-associated genotypes of the commensal skin bacterium Staphylococcus epidermidis.Nature communications · 2018Article
- Development of a High-ThroughputFrontiers in cellular and infection microbiology · 2018Article
- Genomic evolution of Staphylococcus aureus isolates colonizing the nares and progressing to bacteremia.PloS one · 2018Article
- Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections.PloS one · 2017Article
- Comparative genome analysis of Weissella ceti, an emerging pathogen of farm-raised rainbow trout.BMC genomics · 2015Article
- Manipulation of Autophagy in Phagocytes Facilitates Staphylococcus aureus Bloodstream Infection.Infection and immunity · 2015Article
- Prolonged growth of a clinical Staphylococcus aureus strain selects for a stable small-colony-variant cell type.Infection and immunity · 2015Article
- Flipping the switch: tools for detecting small molecule inhibitors of staphylococcal virulence.Frontiers in microbiology · 2014Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The staphylococcal accessory gene regulatory (agr) operon is a well-characterised global regulatory element that is important in the control of virulence gene expression for Staphylococcus aureus, a major human pathogen. Hence, accurate and sensitive measurement of Agr activity is central in understanding the virulence potential of Staphylococcus aureus, especially in the context of Agr dysfunction, which has been linked with persistent bacteraemia and reduced susceptibility to glycopeptide antibiotics. Agr function is typically measured using a synergistic haemolysis CAMP assay, which is believe to report on the level of expression of one of the translated products of the agr locus, delta toxin. In this study we develop a vesicle lysis test (VLT) that is specific to small amphipathic peptides, most notably delta and Phenol Soluble Modulin (PSM) toxins. To determine the accuracy of this VLT method in assaying Agr activity, we compared it to the CAMP assay using 89 clinical Staphylococcus aureus isolates. Of the 89 isolates, 16 were designated as having dysfunctional Agr systems by the CAMP assay, whereas only three were designated as such by VLT. Molecular analysis demonstrated that of these 16 isolates, the 13 designated as having a functional Agr system by VLT transcribed rnaIII and secreted delta toxin, demonstrating they have a functional Agr system despite the results of the CAMP assay. The agr locus of all 16 isolates was sequenced, and only the 3 designated as having a dysfunctional Agr system contained mutations, explaining their Agr dysfunction. Given the potentially important link between Agr dysfunction and clinical outcome, we have developed an assay that determines this more accurately than the conventional CAMP assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.